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活性验证

Recombinant Mouse TNFSF11/RANKL/CD254 Protein (RP02134)

文献引用 (1) 评论 (2) 说明书 收藏

Recombinant Mouse TNFSF11/RANKL/CD254 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Recombinant Mouse TNFSF11/RANKL/CD254 Protein is greater than 95% as determined by SEC-HPLC.

M-CSF (RP01216S, 50 ng/mL) and RANKL (100 ng/mL) induced differentiation of osteoclasts from primary mouse bone marrow cells for 3 days (top); RANKL (100 ng/mL) induced differentiation of osteoclasts from RAW264.7 cells for 5 days (below).

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货号: RP02134
促销价：   ￥780
货    期：现货产品
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详细信息

种属
Mouse
表达宿主
HEK293 cells
Calculated MW
55.63 KD
Observed MW
60-75 KD
标签
N-His＆N-hFC
纯度
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
内毒素
< 0.01 EU/μg of the protein by LAL method
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Mouse TNFSF11/RANKL/CD254 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg72-Asp316) of mouse TNFSF11/RANKL/CD254 (Accession #AAC40113.1) fused with a His and hFc tag at the N-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
M-CSF (50 ng/mL) and RANKL (100 ng/mL) can induced differentiation of osteoclasts from primary mouse bone marrow cells for 3 days ; RANKL (100 ng/mL) can induced differentiation of osteoclasts from RAW264.7 cells for 5 days .
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

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客户数据及评论 (2)

折叠内容
安徽医科大学第一附属医院Jun 092025
评分
Experiment Type
Cell Culture related
Sample
RAW264.7
Description
Recominant Mouse RANKL induced differentiation of RAW264.7 cells into Osteoclast. (Cat. RP02134, 100 ng/mL, 96h)
中国人民解放军陆军军医大学第一附属医院Nov 012024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
Osteoclast induced from primary mouse bone marrow cells

文献引用 (1)

Publishing research using RP02134? Please let us know so that we can cite the reference in this datasheet.

背景信息

Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type II membrane protein that belongs to the tumor necrosis factor family. The receptor activator of nuclear factor-kappaB ligand (RANKL), its cognate receptor RANK, and its natural decoy receptor osteoprotegerin have been identified as the final effector molecules of osteoclastic bone resorption. RANK and RANKL are key regulators of bone remodeling and regulate T cell/dendritic cell communications, and lymph node formation. Moreover, RANKL and RANK are expressed in mammary gland epithelial cells and control the development of a lactating mammary gland during pregnancy. Genetically, RANKL and RANK are essential for the development and activation of osteoclasts and bone loss in response to virtually all triggers tested. Inhibition of RANKL function via the natural decoy receptor osteoprotegerin (OPG, TNFRSF11B) prevents bone loss in postmenopausal osteoporosis and cancer metastases. Importantly, RANKL appears to be the pathogenetic principle that causes bone and cartilage destruction in arthritis. RANK-RANKL signaling not only activates a variety of downstream signaling pathways required for osteoclast development, but crosstalk with other signaling pathways also fine-tunes bone homeostasis both in normal physiology and disease. In addition, RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy.

基因ID
21943
Swiss Prot
O35235-1
别名
ODF; OPGL; RANKL; Ly109l; Trance; TNFSF11

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