Recombinant Human MMP-8 Protein (RP02626)

Recombinant Human MMP-8 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 60-75kDa.

The purity of Human MMP-8 is greater than 95% as determined by SEC-HPLC.

Immobilized Human MMP-8, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC₅₀ of 3.5μg/ml determined by ELISA.

Anti-MMP-8 Antibody captured on CM5 Chip via Protein A can bind Human MMP-8, His Tag with an affinity constant of 1.14 μM as determined in SPR assay (Biacore T200).

Recombinant Human MMP-8 Protein cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RD,Catalog # ES001). The specific activity is >194 pmol/min/µg, as measured under the described conditions.

All(5)|

货号: RP02626

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

51.97 kDa

Observed MW

60-75 kDa

标签

C-His

纯度

> 95% as determined by Tris-Bis PAGE

内毒素

<0.01EU/μg by the LAL method.

制剂

Lyophilized from a 0.2 μm filtered solution of 50mM Tris , 10 mM CaCl2，150 mM NaCl, pH 7.5. Contact us for customized product form or formulation.

描述

Recombinant Human MMP-8 Protein is expressed from Expi293 with His tag at the C-terminal.；It contains Phe21-Gly467.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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背景信息

Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases, including dilated cardiomyopathy (DCM), with the significance of surrogate markers of extracellular matrix (ECM) remodeling. MMP-8 was identified only in myocardiocytes, while MMP-9 and TIMP-2 were present in both myocardiocytes and stroma, but with different intensity. The increasing intensity of MMP-8 and TIMP-2 immunoreactions was significantly associated with low HCS.

基因ID

4317

Swiss Prot

P22894

别名

MMP-8; PMNL-CL; CLG1; MMP8; Collagenase 2; PMNL-CL; HNC

研究领域