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Recombinant Human KIR3DL2/CD158k Protein (RP02706)

Human KIR3DL2 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

The purity of Human KIR3DL2 is greater than 95% as determined by SEC-HPLC.

Immobilized Human KIR3DL2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-KIR3DL2 Antibody, hFc Tag with the EC50 of 0.47μg/ml determined by ELISA.

All(3)|
货号: RP02706
促销价:   ¥2700
货    期:在线咨询货期
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
37.9 kDa
Observed MW
40-70 kDa
标签
C-His&Avi
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per μg by the LAL method.
描述
Recombinant Human KIR3DL2/CD158k Protein is produced by Expi293 cells expression system. The target protein is expressed with sequence (Leu22-Leu339) of Human KIR3DL2/CD158k (Accession #P43630-1) fused with His tag and Avi tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

KIR3DL2 is a member of the killer cell immunoglobulin-like receptor (KIR) family that was initially identified at the surface of natural killer (NK) cells. KIR3DL2, also known as CD158k, is expressed as a disulfide-linked homodimer. Each chain is composed of three immunoglobulin-like domains and a long cytoplasmic tail containing two immunoreceptor tyrosine-based inhibitory motifs.

基因ID
Swiss Prot
别名
NKAT-4; NKAT4; CD158k; CL-5; KIR3DL2; NKAT4A; NKAT4B; p140