Recombinant Mouse GAS-6 Protein (RP02814)

Recombinant Human VHL Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 30 kDa.

The purity of Mouse GAS6 is greater than 95% as determined by SEC-HPLC.

Immobilized Mouse GAS6, His Tag at 5μg/m l (100μl/well) on the plate. Dose response curve for Human AXL, hFc Tag with the EC₅₀ of 3.1ng/ml determined by ELISA (QC Test).

Mouse AXL, His Tag immobilized on CM5 Chip can bind Mouse GAS6, His Tag with an affinity constant of 15.62 nM as determined in SPR assay (Biacore T200) (Routine Test).

All(4)|

货号: RP02814

详细信息

种属

Mouse

表达宿主

HEK293 cells

Calculated MW

73.09 kDa

Observed MW

74-90 kDa

标签

C-His

纯度

> 95% as determined by HPLC

内毒素

<1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Mouse GAS-6 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val28-Pro674) of mouse GAS-6 (Accession #NP_062394.2) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Immobilized Mouse GAS6, His Tag at 5μg/m l (100μl/well) on the plate. Dose response curve for Human AXL, hFc Tag with the EC₅₀ of 3.1ng/ml determined by ELISA (QC Test).
2.Mouse AXL, His Tag immobilized on CM5 Chip can bind Mouse GAS6, His Tag with an affinity constant of 15.62 nM as determined in SPR assay (Biacore T200) (Routine Test).

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Growth arrest-specific 6, also known as Gas6, is a human gene encoding the Gas6 protein, which was originally found to be upregulated in growth-arrested fibroblasts. Gas6 is a member of the vitamin K-dependent family of proteins expressed in many human tissues and regulates several biological processes in cells, including proliferation, survival and migration, by binding to its receptors Tyro3, Axl and Mer (TAM).

基因ID

14456

Swiss Prot

Q61592

别名

GAS6;AXLLG;AXSF