Recombinant Mouse CXCL4/PF-4 Protein (RP03170)

Recombinant Mouse CXCL4/PF-4 Protein 1/FSTL1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 40-45kDa.

The purity of Mouse CXCL4 is greater than 95% as determined by SEC-HPLC.

All(2)|

货号: RP03170

详细信息

种属

Mouse

表达宿主

HEK293 cells

Calculated MW

34.17 kDa

Observed MW

40-45 kDa

标签

C-hFc

纯度

PBS

内毒素

<0.01 EU/ug

制剂

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

描述

Recombinant Mouse CXCL4/PF-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val30-Ser105) of Mouse CXCL4/PF-4 (Accession #NP_064316.1) fused with a hFc at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Human CCL5 (Catalog: RP01750LQ) at 5 μg/mL (100 μL/well) can bind Mouse CXCL4(Catalog: RP03170) with a linear range of 10-252.6 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

基因ID

56744

Swiss Prot

Q9Z126

别名

Pf4; Cxcl4; Scyb4; Platelet factor 4; PF-4; C-X-C motif chemokine 4