Recombinant Mouse CCL12/MCP-5 Protein (RP03278)

说明书收藏

Recombinant Mouse CCL12/MCP-5 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 10-20 kDa.

All(1)|

货号: RP03278

详细信息

种属

Mouse

表达宿主

Yeast

Calculated MW

10.7 kDa

Observed MW

10-20 kDa

标签

C-His

纯度

> 90% by SDS-PAGE.

内毒素

Please contact us for more information.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.

描述

Recombinant Mouse CCL12/MCP-5 Protein is produced by Yeast expression system. The target protein is expressed with sequence (Gly23-Gly104) of mouse CCL12/MCP-5 (Accession #NP_035461.2) fused with His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (0)

折叠内容

背景信息

MCP-5/CCL12 protein promotes CCR2 chemokine receptor binding and contributes to physiological processes such as angiogenesis, inflammation, and monocyte chemotaxis. It is located extracellularly and is widely expressed in tissues such as the central nervous system. CCL12 attracts eosinophils, monocytes, and lymphocytes but not neutrophils, and it is a potent monocyte active chemokine that signals through CCR2, and involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

基因ID

20293

Swiss Prot

Q62401

别名

C-C motif chemokine 12; CCL12; Monocyte chemotactic protein 5; MCP-5; MCP5; Small-inducible cytokine A12; Scya12