Recombinant Human Neutrophil cytosol factor 1/NCF1 Protein (RP03285)

Recombinant Human Neutrophil cytosol factor 1/NCF1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 45-50 kDa.

All(1)|

货号: RP03285

详细信息

种属

Human

表达宿主

E.coli

Calculated MW

45.6 kDa

Observed MW

45-50 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 1.0 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, pH 8.0. Contact us for customized product form or formulation.

描述

Recombinant Human Neutrophil cytosol factor 1/NCF1 Protein is produced by E.coli expression system. The target protein is expressed with sequence (Met1-Val390) of human Neutrophil cytosol factor 1/NCF1 (Accession #P14598) fused with His tag at the C-terminus.

储存

Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Please contact us for reconstitution instructions.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

NCF1 is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This protein is essential for activation of the NADPH oxidase complex, which is essential for superoxide production. It cooperates with NCF2 to form a core association in the complex, interacting with NCF4, CYBB and CYBA. Mutations in the NCF1 gene have been associated with chronic granulomatous disease.

基因ID

653361

Swiss Prot

P14598

别名

NCF1; NOXO2; SH3PXD1A; NCF-47K; NCF-1; p47-phox; Nox organizer 2; Neutrophil cytosol factor 1; Neutrophil NADPH oxidase factor 1; 47 kDa neutrophil oxidase factor; 47 kDa autosomal chronic granulomatous disease protein