Recombinant Human IL-24 Protein (RP03298)

说明书收藏

Recombinant Human IL-24 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35 kDa.

All(1)|

货号: RP03298

详细信息

种属

Human

表达宿主

HEK293 Cells

Calculated MW

19.5 kDa

Observed MW

35 kDa

标签

C-his

纯度

> 94 % as determined by SDS-PAGE

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

描述

Recombinant Human IL-24 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met 1-Leu 207) of Human IL-24 (Accession #NP_001172085.1) fused with His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human IL20 Rα and human IL20 Rβ. The EC50 for this effect is typically 0.05-0.25 ng/mL.

复溶

Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Interleukin-24 (IL-24) also known as Melanoma differentiation-associatedgene 7 protein (MDA-7) is a member of the IL10 family of cytokines. IL24/MDA-7/IL24 can induce apoptosis selectively in various cancer cells.Overexpression of IL-24 leads to elevated expression of several GADDfamily genes, which correlates with the induction of apoptosis. Thephosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), andheat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced bythis gene in melanoma cells, but not in normal immortal melanocytes.Human IL-24/MDA-7/IL24 is secreted by activated peripheral bloodmononuclear cells and is the ligand for two heterodimeric receptors, IL22R1/IL-20R2 and IL-20R1/IL-20R2. Northern blot analysis revealed IL24/MDA-7/IL24 expression in human tissues associated with the immunesystem such as spleen, thymus, peripheral blood leukocytes and normalmelanocytes. IL-24/MDA-7/IL24 binding to either its endogenous receptorson human keratinocytes or to ectopically expressed receptors on babyhamster kidney cells leads to activation of the signal transducers andactivators of transcription.

基因ID

11009

Swiss Prot

Q13007

别名

IL24; MDA7; ST16; Interleukin-24; IL-24; Melanoma differentiation-associated gene 7 protein; MDA-7; Suppression of tumorigenicity 16 protein

研究领域