Recombinant Human CDK4&Cyclin D1 Protein (RP03313LQ)

Recombinant Human CDK4&Cyclin D1 Protein was resolved with SDS-PAGE under reducing (Lane 1) and non-reducing (Lane 2) conditions.

The activity of CDK4/Cyclin D1 is based on the ADP-Glo technology, and the ADP-GLO kinase activity assay quantifies kinase activity by measuring the conversion of ATP to ADP catalyzed by the kinase. Specific reagents are used to convert the ADP in the reaction back to ATP, resulting in the production of a luminescent signal.

All(2)|

货号: RP03313LQ

详细信息

种属

Human

表达宿主

Baculovirus-Insect Cells

Calculated MW

60.3 kDa/36.5 kDa

Observed MW

50-60 kDa/30-40 kDa

标签

N-GST (CDK4) & N-Flag-His (Cyclin D1)

纯度

> 90% by SDS-PAGE and HPLC

内毒素

< 1.0 EU/μg of the protein by LAL method

制剂

Supplied as a 0.22 μm filtered solution in 50 mM Tris-HCl, 150 mM NaCl, 5% glycerol. (pH 7.5). Contact us for customized product form or formulation.

描述

Recombinant Human CDK4&Cyclin D1 Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Met1-Glu303 (CDK4) & Met1-Ile295 (Cyclin D1)) of Human CDK4&CCND1 (Accession #P11802&P24385) fused with a N-GST (CDK4) & N-Flag-His (Cyclin D1) tag.

储存

Store at -70°C. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
Aliquots below 10 μL are not advisable. Product must not be stored in diluted solutions. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

复溶

Please use running water to thaw it quickly.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

CDK4 also known as cyclin-dependent kinase 4 or cell division protein kinase 4 is a member of the Ser/Thr protein kinase family. The activity of CDK4 is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16. CDK4/Cyclin D complex phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition.

基因ID

1019&595

Swiss Prot

P11802&P24385

别名

CDK4; PSK-J3; Cyclin-dependent kinase 4; Cyclin D1; CCND1; BCL1; PRAD1; G1/S-specific cyclin-D1

研究领域