Recombinant Human CDK6&Cyclin D1 Protein (RP03316LQ)

Recombinant Human CDK6&Cyclin D1 Protein was resolved with SDS-PAGE under reducing (Lane 1) and non-reducing (Lane 2) conditions.

The activity of CDK6/Cyclin D1 is based on the MSA technology, and the content and ratio of the substrate and the product are directly separated and detected in real time and dynamically by the different migration rates of the substrate and the product after the enzymatic reaction.

All(2)|

货号: RP03316LQ

详细信息

种属

Human

表达宿主

Baculovirus-Insect Cells

Calculated MW

63.5 kDa/36.5 kDa

Observed MW

50-60 kDa/30-40 kDa

标签

N-GST (CDK6) & N-Flag-His (Cyclin D1)

纯度

> 90% by SDS-PAGE and HPLC

内毒素

< 1.0 EU/μg of the protein by LAL method

制剂

Supplied as a 0.22 μm filtered solution in 50 mM HEPES, 150 mM NaCl, 5% glycerol. (pH 7.5). Contact us for customized product form or formulation.

描述

Recombinant Human CDK6&Cyclin D1 Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Met1-Ala326 (CDK6) & Met1-Ile295 (Cyclin D1)) of Human CDK6&CCND1 (Accession #Q00534&P24385) fused with a N-GST (CDK6) & N-Flag-His (Cyclin D1) tag.

储存

Store at -70°C. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
Aliquots below 10 μL are not advisable. Product must not be stored in diluted solutions. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

复溶

Please use running water to thaw it quickly.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

CDK6 also known as cell division protein kinase 6 is a member of the cyclin-dependent kinase family of Ser/Thr protein kinases. It is positively regulated primarily by its union to the D cyclins D1, D2 and D3. The activity of CDK6 first appears in mid-G1 phase, which is controlled by the Cyclin D and members of INK4 family of CDK inhibitors. CDK6, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor Retinoblastoma protein making CDK6 an important protein in cancer development.

基因ID

1021&595

Swiss Prot

Q00534&P24385

别名

CDK6; CDKN6; Cyclin-dependent kinase 6; Cyclin D1; CCND1; BCL1; PRAD1; G1/S-specific cyclin-D1