Recombinant Human MELK Protein (RP03336LQ)

Recombinant Human MELK Protein was resolved with SDS-PAGE under reducing (Lane 1) and non-reducing (Lane 2) conditions.

The activity of MELK is based on the MSA technology, and the content and ratio of the substrate and the product are directly separated and detected in real time and dynamically by the different migration rates of the substrate and the product after the enzymatic reaction.

All(2)|

货号: RP03336LQ

详细信息

种属

Human

表达宿主

E. coli

Calculated MW

39.7 kDa

Observed MW

30-40 kDa

标签

N-His

纯度

> 90% by SDS-PAGE and HPLC

内毒素

< 1.0 EU/μg of the protein by LAL method

制剂

Supplied as a 0.22 μm filtered solution in 50 mM Tris-HCl, 200 mM NaCl, 20% glycerol, 1 mM DTT. (pH 7.5). Contact us for customized product form or formulation.

描述

Recombinant Human MELK Protein is produced by E. coli expression system. The target protein is expressed with sequence (Asp3-Val330) of Human MELK (Accession #Q14680) fused with a N-His tag.

储存

Store at -70°C. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
Aliquots below 10 μL are not advisable. Product must not be stored in diluted solutions. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

The activity of MELK is based on the MSA technology, and the content and ratio of the substrate and the product are directly separated and detected in real time and dynamically by the different migration rates of the substrate and the product after the enzymatic reaction.

复溶

Please use running water to thaw it quickly.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Maternal embryonic leucine zipper kinase (MELK) is a serine/threonine kinase belonging to the family of AMPK/Snf1 protein kinases. MELK was first identified present as maternal mRNA in mouse embryos, and has been shown to interact with CDC25B. MELK expression is elevated in a number of cancers and is an active research target for pharmacological inhibition.

基因ID

9833

Swiss Prot

Q14680

别名

MELK; KIAA0175; pEg3 kinase; hPK38; Maternal embryonic leucine zipper kinase

研究领域