Recombinant Human PIM2 Protein was resolved with SDS-PAGE under reducing (Lane 1) and non-reducing (Lane 2) conditions.
The activity of PIM2 is based on the MSA technology, and the content and ratio of the substrate and the product are directly separated and detected in real time and dynamically by the different migration rates of the substrate and the product after the enzymatic reaction.
Serine/threonine-protein kinase Pim-2 is encoded by the PIM2 (Proviral Integrations of Moloney virus 2) gene in human. PIM2 is expressed with high levels in the brain and lymphoid cells. Like PIM1, PIM2 shows a bi-lobal kinase architecture with a constitutively active closed conformation. The main chain of both molecules is identical with the exception of two flexible regions in the N-terminal lobe. PIM2 has roles in cell growth, proliferation, apoptosis, and regulation of signal transduction cascades. In clinical studies, PIM2 may be an important kinase in the phosphorylation of 4E-BP1, that is commonly found in cancers and contributes to the sustained translation of malignancy related transcripts. As a result, PIM2 may be an attractive target for acute myeloid leukemia.
