![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_ChIP-seq_01.jpg?t=1713323641)
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 105 K562 cells with 1 ug Histone H3 Rabbit mAb,along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of Histone H3 in representative gene loci (RPL30), as shown in figure.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-04_WB_01.jpg?t=1713323641)
Western blot analysis of various lysates using Histone H3 Rabbit mAb (A22348) at 1:4000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit (RM00020). Exposuretime: 10s.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IHC_01.jpg?t=1713323641)
Immunohistochemistry analysis of Histone H3 in paraffin-embedded human breast cancer using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IHC_02.jpg?t=1713323641)
Immunohistochemistry analysis of Histone H3 in paraffin-embedded human colon using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IHC_03.jpg?t=1713323641)
Immunohistochemistry analysis of Histone H3 in paraffin-embedded human kidney using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IHC_04.jpg?t=1713323641)
Immunohistochemistry analysis of Histone H3 in paraffin-embedded human liver cancer using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IHC_05.jpg?t=1713323641)
Immunohistochemistry analysis of Histone H3 in paraffin-embedded human placenta using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IHC_06.jpg?t=1713323641)
Immunohistochemistry analysis of Histone H3 in paraffin-embedded human spleen using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IHC_07.jpg?t=1713323641)
Immunohistochemistry analysis of Histone H3 in paraffin-embedded human testis using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IHC_08.jpg?t=1713323641)
Immunohistochemistry analysis of Histone H3 in paraffin-embedded human thyroid cancer using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IHC_09.jpg?t=1713323641)
Immunohistochemistry analysis of Histone H3 in paraffin-embedded human tonsil using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IHC_10.jpg?t=1713323641)
Immunohistochemistry analysis of Histone H3 in paraffin-embedded mouse liver using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IHC_11.jpg?t=1713323641)
Immunohistochemistry analysis of Histone H3 in paraffin-embedded mouse lung using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IHC_12.jpg?t=1713323641)
Immunohistochemistry analysis of Histone H3 in paraffin-embedded rat colon using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IF_01.jpg?t=1713323641)
Immunofluorescence analysis of HeLa cells using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IF_02.jpg?t=1713323641)
Immunofluorescence analysis of NIH/3T3 cells using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A22348_ARC50519-03_IF_03.jpg?t=1713323641)
Immunofluorescence analysis of PC-12 cells using Histone H3 Rabbit mAb (A22348) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.