ARHGEF1 Knockout 293T Cell Lysate, Homozygous (RM02495)

Genome sequence analysis of PCR products from parental (WT) and ARHGEF1 knockout (KO) 293T cells, using sanger sequencing.

All(1)|

货号: RM02495

详细信息

靶点

ARHGEF1

细胞系

293T

突变描述

ARHGEF1 Knockout 293T Cell Line is engineered from 293T cell line with Gene-Editing technology.
Allele-1:exon6 was deleted
Allele-2:exon6 was deleted

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

基因ID

9138

Swiss Prot

Q92888

别名

GEF1; LBCL2; LSC; P115-RHOGEF; SUB1.5

研究领域