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Recombinant Human CD20 Protein-VLP (RP00583)

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The purity of Human CD20 VLP is greater than 95% as determined by SEC-HPLC.

Immobilized Human CD20 VLP at 5 μg/mL (100 μL/Well) on plate on the plate. Dose response curve for Ofatumumab, hFc Tag with the EC50 of 10.5ng/mL determined by ELISA (QC Test).

Immobilized Human CD20 VLP at 5 μg/mL (100 μL/Well) on plate on the plate. Dose response curve for Rituximab, hFc Tag with the EC50 of 11.4ng/mL determined by ELISA (QC Test).

Immobilized Human CD20 VLP at 5 μg/mL (100 μL/well) on the plate. Dose response curve for Obinutuzumab, hFc Tag with the EC50 of 1.7ng/mL determined by ELISA.

All(4)|
货号: RP00583
促销价：   ￥12000
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详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human CD20 Protein-VLP is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Pro297) of Human CD20-VLP (Accession #P11836-1) fused with No Tag.
标签
No Tag
纯度
> 95% by HPLC
内毒素
< 1 EU/μg of the protein by LAL method
制剂
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.Notice: If you need it for immunization, Do Not use any adjuvant.
储存
Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R , CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.

基因ID
931
Swiss Prot
P11836-1
别名
MS4A1; CD20; MS4A-1; B1; Bp35; CD20 receptor; CD20S7; CVID5; LEU-16; Ly-44; MS4A2; S7
研究领域
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