Recombinant Human Prolyl endopeptidase FAP Protein is produced by Baculovirus-Infected Sf9 Cells expression system. The target protein is expressed with sequence (Arg27-Asp760) of human FAP (Accession #NP_004451.2) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized Human FAP at 1 μg/mL (100 μL/well) can bind FAP Rabbit mAb with a linear range of 0.03-1.77 ng/mL. 2.Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC).The specific activity is >2314 pmol/min/μg.
制剂
Supplied as a 0.22 μm filtered solution in 16mM Tris,240mM Nacl and 20% glycerol,pH7.5.Contact us for customized product form or formulation.
储存
This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
