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活性验证

Recombinant SARS-CoV-2 Spike RBD(B.1.640.2/IHU) Protein (RP02104)

文献引用 (1) 说明书 收藏

SARS-Cov-2 Spike RBD (B.1.640.2/IHU) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of SARS-Cov-2 Spike RBD (B.1.640.2/IHU) is greater than 95% as determined by SEC-HPLC.

Immobilized SARS-Cov-2 Spike RBD (B.1.640.2/IHU) , His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 41.3ng/ml determined by ELISA.

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货号: RP02104
促销价：   ￥3000
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详细信息

种属
SARS-CoV-2
表达宿主
HEK293 cells
Calculated MW
40 kDa
标签
C-His
纯度
>95% by SDS-PAGE;> 95% by HPLC
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant SARS-CoV-2(2019-nCoV) Spike RBD(B.1.640.2/IHU)-His Protein is produced by Expi293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541(R346S, N394S, Y449N, E484K, F490S, N501Y)) of SARS-COV-2(2019-nCoV) Spike RBD(B.1.640.2/IHU)-His (Accession #QHD43416.1) fused with His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Immobilized SARS-Cov-2 Spike RBD(B.1.640.2/IHU), His Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 41.3 ng/mL determined by ELISA.
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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文献引用 (1)

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Publishing research using RP02104? Please let us know so that we can cite the reference in this datasheet.

背景信息

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

基因ID
43740568
Swiss Prot
QHD43416.1
别名
Envelope;SARS-CoV-2 Spike RBD (N501Y);Spike;Spike ECD;Spike RBD;Spike S1;Spike S2;Spike S2 ECD;S1-RBD protein;NCP-CoV RBD Protein;novel coronavirus RBD Protein;2019-nCoV RBD Protein;S glycoprotein Subunit1 RBD Protein
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