Recombinant Human IL-17A&IL-17F Protein (RP02627)

Human IL-17A&F on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%

The purity of Human IL-17A&F is greater than 95% as determined by SEC-HPLC.

Immobilized Human IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC₅₀ of 34.0ng/ml determined by ELISA.

All(3)|

货号: RP02627

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

15.1 kDa (IL-17A), 15.7 kDa (IL-17F)

Observed MW

16 kDa(IL-17A) , 27 kDa(IL-17F)

标签

C-His

纯度

> 95% as determined by Tris-Bis PAGE；> 95% as determined by HPLC

内毒素

Less than 1EU per μg by the LAL method.

描述

Recombinant Human IL-17A&IL-17F Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly24-Ala155)&(Arg31-Gln163) of Human IL-17A&IL-17F (Accession #) fused with His tag at the C-terminal.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Interleukin?17A (IL?17A), also known as CTLA?8, is a 15?20 kDa glycosylated cytokine that plays an important role in anti?microbial and chronic inflammation. The six IL?17 cytokines (IL?17A?F) are encoded by separate genes but adopt a conserved cystine knot fold .IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC . Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.

基因ID

3605&112744

Swiss Prot

Q16552-1(IL-17A)&Q96PD4(IL-17F)

别名

IL-17A;Interleukin-17A;CTLA-8;IL-17;IL-17F;IL24;ML-1;Interleukin-17F

研究领域