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Recombinant Mouse Artemin/ARTN Prptein (RP02718)

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Mouse ARTN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Mouse ARTN is greater than 95% as determined by SEC-HPLC.

Immobilized Mouse GFRA3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse ARTN, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA.

All(3)|
货号: RP02718
促销价：   ￥4000
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详细信息

种属
Mouse
表达宿主
HEK293 cells
描述
Recombinant Mouse Artemin/ARTN Prptein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala112-Gly224) of Mouse Artemin/ARTN (Accession #) fused with hFc tag at the N-terminal.
标签
N-hFc
纯度
> 95% as determined by Tris-Bis PAGE；> 95% as determined by HPLC
内毒素
Less than 1EU per μg by the LAL method.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The majorhanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons.

基因ID
11876
Swiss Prot
Q9Z0L2-1
别名
Artemin; Enovin; Neublastin; ARTNE; EVN; ARTN; NBN
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