Recombinant Human TUBB4A Protein (RP02833)

说明书收藏

Recombinant Human TUBB4A Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 58 KDa.

All(1)|

货号: RP02833

详细信息

种属

Human

表达宿主

E. coli

Calculated MW

51.7 kDa

Observed MW

58 kDa

标签

N-His

纯度

> 90% as determined by by reducing SDS-PAGE.

内毒素

<1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of 20mM Tris-HCl, 50mM NaCl, 2M Urea, pH8.0.

描述

Recombinant Human TUBB4A Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ala444) of human TUBB4A (Accession #NP_006078.2) fused with a 6×His tag at the N-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Tubulin Beta-4A Chain (TUBB4A) is a cytoplasmic peptide containing 444 amino acids. TUBB4A is a member of the Tubulin family. Tubulin is the major constituent of microtubules. Tubulin is a dimer composed of one alpha and one beta tubulin molecule; there are many forms of beta tubulins, Beta II and Beta IV Tubulin are ubiquitously expressed. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

基因ID

10382

Swiss Prot

P04350

别名

Tubulin Beta-4A Chain; Tubulin 5 Beta; Tubulin Beta-4 Chain; TUBB4A; TUBB4; TUBB5

研究领域