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Recombinant Human FLAP Protein (RP02837)

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Recombinant Human FLAP/ALOX5AP Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 19 kDa.

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货号: RP02837
促销价：   ￥5200
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详细信息

种属
Human
表达宿主
Baculovirus-Insect Cells
描述
Recombinant Human FLAP/ALOX5AP Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Met1-Pro161) of human FLAP/ALOX5AP (Accession #NP_001620.2) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 90% by SDS-PAGE.
内毒素
<1EU/μg
制剂
Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl, 10% gly, pH 7.4, 0.5% triton, 5mM DTT.
储存
Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP), also known as FLAP, belongs to the MAPEG family. ALOX5AP/FLAP is an essential partner of 5-LO for this process. The FLAP (ALOX5AP) gene has been linked to risk for myocardial infarction, stroke and restenosis, reigniting pharmaceutical interest in this target. It had been found that ALOX5AP/FLAP is a key enzyme in leukotriene formation, in both human pulmonary microvascular endothelial cells and a transformed human brain endothelial cell line. In addition, the protein FLAP has recently been identified as an emerging target in metabolic disease. In fact, FLAP is overexpressed in the adipose tissue of patients and experimental animals with obesity.

基因ID
241
Swiss Prot
P20292
别名
Arachidonate 5-lipoxygenase-activating protein; ALOX5AP; FLAP; MK-886-binding protein
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