Recombinant Human p21-activated kinase 6/PAK6 Kinase was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
The activity of PAK6 is based on the MSA technology, and the content and ratio of the substrate and the product are directly separated and detected in real time and dynamically by the different migration rates of the substrate and the product after the enzymatic reaction.
Serine/threonine-protein kinase PAK 6 (PAK6) belongs to the Group II PAK family members. Following the general structural organization of the group II PAKs, PAK6 also contains a kinase, and a GTPase interacting domain. The proteins of PAK family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK6 kinase activity is positively regulated by androgen receptor, p38MAPK, 5-fluorouracil and atypical Rho family GTPase Chp/RhoV. PAK6 expression is negatively regulated by miR-328, miR-429 and miR-23a.
