Western blot analysis of lysates from Mouse lung using JAK1 Rabbit mAb (A11963) at 1:1000 dilution incubated overnight at 4℃.
Immunofluorescence analysis of paraffin-embedded rat spleen using SHP1 Rabbit mAb (A19111) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
*Immunofluorescence analysis of paraffin-embedded mouse spleen using SHP1 Rabbit mAb (A19111) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
*Western blot analysis of lysates from Raji cells, using SHP1 Rabbit mAb (A19111) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of various lysates using Phospho-STAT1-Y701 Rabbit mAb (AP0054) at 1:1000 dilution incubated overnight at 4℃. HeLa cells, NIH/3T3 cells and PC-12 cells were treated with TNF-α (100 ng/mL) at 37℃ for 30 minutes.
Western blot analysis of lysates from K-562 cells, using SOCS2 Rabbit mAb (A9190) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s..
*Immunohistochemistry analysis of paraffin-embedded Rat lung using SOCS2 Rabbit mAb (A9190) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Western blot analysis of various lysates using Phospho-STAT3-S727 Rabbit mAb (AP0715) at 1:10000 dilution incubated overnight at 4℃. HeLa cells and NIH/3T3 cells were treated with λPP (2 U/μL) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45 s..
*Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-STAT3-S727 Rabbit mAb (AP0715) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining..
*Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-STAT3-S727 Rabbit mAb (AP0715) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining..
*Immunohistochemistry analysis of paraffin-embedded Rat colon using Phospho-STAT3-S727 Rabbit mAb (AP0715) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining..
*Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-STAT3-S727 Rabbit mAb (AP0715) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Western blot analysis of various lysates using STAT4 Rabbit mAb (A4523) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s..
*Immunofluorescence analysis of C6 cells using STAT4 Rabbit mAb (A4523) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining..
*Immunofluorescence analysis of NIH-3T3 cells using STAT4 Rabbit mAb (A4523) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
