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VEGFA Rabbit pAb (A12303)

文献引用 (99) 评论 (1) 说明书 收藏

Western blot analysis of various lysates, using VEGFA Rabbit pAb (A12303) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.

Immunofluorescence analysis of NIH/3T3 cells using VEGFA Rabbit pAb (A12303) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

All(2)|
货号: A12303

促销价：   ￥1150
货    期：现货产品
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详细信息

推荐稀释比
  • WB1:500 - 1:1000
  • IF/ICC1:50 - 1:200
  • ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
浓度查询

请输入产品标签上的lot号，例如4000000001

反应物种
Human, Mouse, Rat
同种型
IgG
理论分子量
27kDa
实际分子量
16kDa/20kDa/23kDa/26kDa
产品形式
Liquid
偶联物
Unconjugated
存储缓冲液
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
阳性样本
U-87MG,K-562,SH-SY5Y,Mouse lung,Rat lung
细胞定位
Secreted.
纯化方式
Affinity purification

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用户验证的应用

应用及物种
  • IHC (Mouse bone tissueHomo sapiens , Mus musculus , Homo sapiens , Gallus gallus , Rattus norvegicus , Homo sapiens , Mus musculus)
  • ELISA (Mus musculus , Homo sapiens , Mus musculus)
  • IF (Homo sapiens , Rattus norvegicus , Mus musculus , Gallus gallus)
  • WB (Mus musculus , Homo sapiens , Mus musculus , Rattus norvegicus , Homo sapiens , Gallus gallus , Neovison vison , Sus scrofa , Oryctolagus cuniculus)
  • RT-qPCR (Mus musculus)
  • qPCR (Mus musculus)
  • IP (Homo sapiens)

客户数据及评论 (1)

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CustomerSep 022017
评分
Experiment Type
Immunohistochemistry(IHC)
Sample
Mouse bone tissue
Description
Immunohistochemistry of Mouse bone tissue using VEGF antibody (A12303) at 1:100 dilution.The staining is perfect.

文献引用 (99)

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Publishing research using A12303? Please let us know so that we can cite the reference in this datasheet.

背景信息

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. The levels of VEGF are increased during infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), thus promoting inflammation by facilitating recruitment of inflammatory cells, and by increasing the level of angiopoietin II (Ang II), one of two products of the SARS-CoV-2 binding target, angiotensin-converting enzyme 2 (ACE2). In turn, Ang II facilitates the elevation of VEGF, thus forming a vicious cycle in the release of inflammatory cytokines.

RRID
AB_2759160
基因ID
7422
Swiss Prot
P15692
别名
VPF; VEGF; MVCD1; VEGFA

抗原信息

免疫原
Recombinant fusion protein containing a sequence corresponding to amino acids 91-191 of human VEGFA (NP_001165097.1).
序列
GLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
进行序列比对
研究领域

关联靶点

以VEGFA为关键词搜索文献记录，得到上述基因与VEGFA的研究紧密相关，排序顺序根据研究相关程度由高到低决定。（所显示数字代表两者共同出现的已发表文献数量）
◀1/▶

关联领域

以VEGFA为关键词搜索文献记录，得到上述相关热词信息，可反映出VEGFA的研究趋势与热点。（关联领域与基因名称的距离代表其研究热度）

* 该数据来源于赛特新思(citexs.com)
来自于发表文献，仅供参考所用，ABclonal不承担任何责任。

暂无以VEGFA为关键词搜索的文献记录。

该服务由赛特新思提供，上述关联分析来自已发表文献，仅供参考所用，ABclonal不承担任何责任。

FAQ

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ABclonal公司的抗体，可以回收利用几次？

首先，一般抗体不推荐客户回收利用，抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，ABclonal公司也做过一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定。
注：我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存，效价高的抗体可至少保存1周，至少重复利用3次。

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ABclonal作为国产抗体品牌的优势？

武汉爱博泰克生物（ABclonal）科技有限公司是国产品牌，她成立于2011年，公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心，凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士，组成我们的科学家团队，通过聚焦抗体与酶核心技术，致力于打破国际技术的垄断，将公司打造成为科研工具和诊断原料的国内领导品牌，乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台，同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台，我们对每一支自产的抗体进行了严格的检测。当然，我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势：1，严自检，保质量；2产品多，指标全；3，价格低，货期短。注：ABclonal抗体价格体系详情见附件

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ABclonal抗体成分？

ABclonal抗体成分在平时工作当中，常会有客户咨询我们的抗体用的什么buffer进行保存，一般来说，我们的buffer的成分是：PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油；也有一些是PBS含0.03%的proclin300，50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮（MCI）和5-氯-2-甲基-4-异噻唑啉-3-酮（CMCI）。ProClin生物灭活剂能够迅速穿透细胞膜，抑制对细胞呼吸至关重要的特定酶，因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。

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实验方案

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