Western blot analysis of various lysates using [KD Validated] Vimentin Rabbit mAb (A19607) at 1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Western blot analysis of lysates from wild type (WT) and Vimentin knockdown (KD) 293T cells using [KD Validated] Vimentin Rabbit mAb (A19607) at 1:20000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.
Confocal imaging of C2C12 cells using [KD Validated] Vimentin Rabbit mAb (A19607,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of C6 cells using [KD Validated] Vimentin Rabbit mAb (A19607,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of paraffin-embedded Rat kidney tissue using [KD Validated] Vimentin Rabbit mAb (A19607, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of HeLa cells using [KD Validated] Vimentin Rabbit mAb (A19607,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of paraffin-embedded Rat testis tissue using [KD Validated] Vimentin Rabbit mAb (A19607, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] Vimentin Rabbit mAb (A19607) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] Vimentin Rabbit mAb (A19607) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] Vimentin Rabbit mAb (A19607) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] Vimentin Rabbit mAb (A19607) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using [KD Validated] Vimentin Rabbit mAb (A19607) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] Vimentin Rabbit mAb (A19607) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
请输入产品标签上的lot号,例如4000000001
展开
Publishing research using A19607? Please let us know so that we can cite the reference in this datasheet.
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Journal
Developmental Cell
Journal
The Science of the total environment
Journal
Bioengineering & translational medicine
Journal
Frontiers in immunology
Journal
International journal of biological macromolecules
Journal
Cellular and molecular life sciences : CMLS
Journal
International journal of nanomedicine
Journal
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Journal
Journal of translational medicine
Journal
Journal of translational medicine
Journal
Oxidative medicine and cellular longevity
Journal
Oxidative medicine and cellular longevity
Journal
Clinical epigenetics
Journal
Cell death discovery
Journal
Cell death discovery
Journal
Phytomedicine : international journal of phytotherapy and phytopharmacology
Journal
Phytomedicine : international journal of phytotherapy and phytopharmacology
Journal
Journal of cellular physiology
Journal
Cancer cell international
Journal
Human reproduction (Oxford, England)
Journal
International journal of molecular sciences
Journal
International journal of molecular sciences
Journal
Frontiers in bioengineering and biotechnology
Journal
Frontiers in pharmacology
Journal
Frontiers in pharmacology
Journal
Cancer Gene Ther
Journal
The American journal of pathology
Journal
Frontiers in oncology
Journal
Frontiers in aging neuroscience
Journal
Molecular medicine (Cambridge, Mass.)
Journal
Int J Biochem Cell Biol
Journal
Frontiers in pharmacology
Journal
Journal of cellular and molecular medicine
Journal
Pharmaceuticals (Basel, Switzerland)
Journal
Journal of ethnopharmacology
Journal
Cell cycle (Georgetown, Tex.)
Journal
Chemico-biological interactions
Journal
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
Journal
Scientific reports
Journal
Cellular signalling
Journal
Cellular signalling
Journal
Cellular signalling
Journal
Cellular signalling
Journal
Cellular signalling
Journal
Cellular Signalling
Journal
Environmental toxicology
Journal
Cell biology international
Journal
The journal of pathology. Clinical research
Journal
Onco Targets Ther
Journal
Drug design, development and therapy
Journal
Experimental cell research
Journal
Oncology reports
Journal
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]
Journal
Cell Biochem Funct
Journal
Journal of applied toxicology : JAT
Journal
DNA and cell biology
Journal
DNA and cell biology
Journal
Biochemical and biophysical research communications
Journal
Biochemical and biophysical research communications
Journal
Journal of cardiovascular pharmacology
Journal
World journal of surgical oncology
Journal
Frontiers in bioscience (Landmark edition)
Journal
Histochemistry and cell biology
Journal
Acta biochimica Polonica
Journal
Hematology (Amsterdam, Netherlands)
Journal
Biological & pharmaceutical bulletin
Journal
Biological & pharmaceutical bulletin
Journal
International journal of ophthalmology
Journal
Open medicine (Warsaw, Poland)
Journal
Molecular biomedicine
Journal
Frontiers in bioscience (Landmark edition)
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
* 该数据来源于赛特新思(citexs.com)
来自于发表文献,仅供参考所用,ABclonal不承担任何责任。
暂无以VIM为关键词搜索的文献记录。
该服务由赛特新思提供,上述关联分析来自已发表文献,仅供参考所用,ABclonal不承担任何责任。
首先,一般抗体不推荐客户回收利用,抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,ABclonal公司也做过一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定。
注:我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存,效价高的抗体可至少保存1周,至少重复利用3次。
武汉爱博泰克生物(ABclonal)科技有限公司是国产品牌,她成立于2011年,公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心,凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士,组成我们的科学家团队,通过聚焦抗体与酶核心技术,致力于打破国际技术的垄断,将公司打造成为科研工具和诊断原料的国内领导品牌,乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台,同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台,我们对每一支自产的抗体进行了严格的检测。当然,我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势:1,严自检,保质量;2产品多,指标全;3,价格低,货期短。注:ABclonal抗体价格体系详情见附件
ABclonal抗体成分在平时工作当中,常会有客户咨询我们的抗体用的什么buffer进行保存,一般来说,我们的buffer的成分是:PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油;也有一些是PBS含0.03%的proclin300,50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮(MCI)和5-氯-2-甲基-4-异噻唑啉-3-酮(CMCI)。ProClin生物灭活剂能够迅速穿透细胞膜,抑制对细胞呼吸至关重要的特定酶,因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。