The m6A rabbit monoclonal antibody (4µg,A19841) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).Oligomer 4 - N6-methyladenosine (m6A-UAACUGGACCGAAUGG-Biotin)Oligomer 5 - N6-methyladenosine (AUAACUGG-m6A-CCGAAUGG-Biotin)Oligomer 6 - unmodified adenosine (AUAACUGGACCGAAUGG-Biotin)
The m6A rabbit monoclonal antibody (A19841) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.Oligomer 8 - ATAACTGG-m6A-CCGAATGGOligomer 9 - ATAACTGGACCGAATGGOligomer 10 - AAAAAAAAAAAAAAAA-biotin.
U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 °C for the indicated time, and stained for m6A rabbit monoclonal antibody (A19841). DAPI, 4′,6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel,peaking at 2 min after irradiation and diminishing over the following 8 min.
Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)