![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_ChIP-seq_01.jpg?t=1721355842)
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ K562 cells with 1 μg Acetyl-Histone H3-K18 Rabbit mAb (A20735), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of H3K18me1 in representative gene loci (RPL30), as shown in figure.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_ChIP-seq_02.jpg?t=1721355842)
Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells and Acetyl-Histone H3-K18 Rabbit mAb (A20735). The ChIP sequencing results indicate the enrichment pattern of Acetyl-Histone H3-K18 in selected genomic region and representative gene loci (RPL30), as shown in figure.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC2680_WB_01.jpg?t=1721355842)
Western blot analysis of various lysates using Acetyl-Histone H3-K18 Rabbit mAb (A20680) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_DotBlot_01.jpg?t=1721355842)
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at 1:100000 dilution.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_IHC_01.jpg?t=1721355842)
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_IHC_02.jpg?t=1721355842)
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_IHC_03.jpg?t=1721355842)
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_IHC_04.jpg?t=1721355842)
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_IHC_05.jpg?t=1721355842)
Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_IHC_06.jpg?t=1721355842)
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_IHC_07.jpg?t=1721355842)
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_IHC_08.jpg?t=1721355842)
Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_IHC_09.jpg?t=1721355842)
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_IHC_10.jpg?t=1721355842)
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_IHC_11.jpg?t=1721355842)
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_IHC_12.jpg?t=1721355842)
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC53056-01_IHC_13.jpg?t=1721355842)
Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Acetyl-Histone H3-K18 Rabbit mAb (A20735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A20735_ARC2680_ChIP_01.jpg?t=1721355842)
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K18 Rabbit mAb (A20735) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.