
Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from HeLa using 5 μg of DiMethyl-Histone H3-K4 Rabbit mAb(A22143). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K4 in the representative genomic region surrounding GAPDH gene.

Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from HeLa using 5 μg of DiMethyl-Histone H3-K4 Rabbit mAb (A22143). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K4 across chromosome 12 (upper panel) and the genomic region encompassing GAPDH, a representative gene enriched in DiMethyl-Histone H3-K4 (lower panel).

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ HeLa with 1 μg of DiMethyl-Histone H3-K4 Rabbit mAb (A22143), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of DiMethyl-Histone H3-K4 across chromosome 12 (upper panel) and the genomic region encompassing GAPDH, a representative gene enriched in DiMethyl-Histone H3-K4 (lower panel).

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ HeLa cells with 1 μg of DiMethyl-Histone H3-K4 Rabbit mAb (A22143), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of DiMethyl-Histone H3-K4 around GAPDH gene.

Western blot analysis of various lysates using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at 1:3000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): H3 proteinExposure time: 30 s.

Dot-blot analysis of all sorts of peptides using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at 1:5000 dilution.

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat pancreas tissue using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Confocal imaging of HeLa cells using DiMethyl-Histone H3-K4 Rabbit pAb (A22143, dilution 1:500) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of NIH/3T3 cells using DiMethyl-Histone H3-K4 Rabbit pAb (A22143, dilution 1:500) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of PC-12 cells using DiMethyl-Histone H3-K4 Rabbit pAb (A22143, dilution 1:500) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Immunoprecipitation of DiMethyl-Histone H3-K4 from 600 µg extracts of HeLa cells was performed using 3 µg of DiMethyl-Histone H3-K4 Rabbit mAb (A22143). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at a dilution of 1:10000.

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using DiMethyl-Histone H3-K4 Rabbit mAb (A22295) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.