Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from HeLa using 5 μg of DiMethyl-Histone H3-K4 Rabbit mAb（A22143）. DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K4 in the representative genomic region surrounding GAPDH gene.
Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from HeLa using 5 μg of DiMethyl-Histone H3-K4 Rabbit mAb (A22143). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K4 across chromosome 12 (upper panel) and the genomic region encompassing GAPDH, a representative gene enriched in DiMethyl-Histone H3-K4 (lower panel).
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ HeLa with 1 μg of DiMethyl-Histone H3-K4 Rabbit mAb (A22143), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of DiMethyl-Histone H3-K4 across chromosome 12 (upper panel) and the genomic region encompassing GAPDH, a representative gene enriched in DiMethyl-Histone H3-K4 (lower panel).
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ HeLa cells with 1 μg of DiMethyl-Histone H3-K4 Rabbit mAb (A22143), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of DiMethyl-Histone H3-K4 around GAPDH gene.
Western blot analysis of lysates from C6 cells using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at 1:10000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.
Western blot analysis of various lysates using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at 1:10000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): H3 proteinExposure time: 20s.
Dot-blot analysis of all sorts of peptides using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at 1:5000 dilution.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at a dilution of 1:1500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at a dilution of 1:1500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at a dilution of 1:1500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at a dilution of 1:1500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at a dilution of 1:1500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Confocal imaging of HeLa cells using DiMethyl-Histone H3-K4 Rabbit mAb (A22143,dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Immunoprecipitation of DiMethyl-Histone H3-K4 Rabbit from 600 µg extracts of HeLa cells was performed using 3 µg of DiMethyl-Histone H3-K4 Rabbit mAb (A22143). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at a dilution of 1:10000.
Immunoprecipitation of DiMethyl-Histone H3-K4 from 600 µg extracts of HeLa cells was performed using 3 µg of DiMethyl-Histone H3-K4 Rabbit mAb (A22143). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using DiMethyl-Histone H3-K4 Rabbit mAb (A22143) at a dilution of 1:10000.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using DiMethyl-Histone H3-K4 Rabbit mAb (A22295) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
首先，一般抗体不推荐客户回收利用，抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，ABclonal公司也做过一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定。
注：我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存，效价高的抗体可至少保存1周，至少重复利用3次。
武汉爱博泰克生物（ABclonal）科技有限公司是国产品牌，她成立于2011年，公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心，凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士，组成我们的科学家团队，通过聚焦抗体与酶核心技术，致力于打破国际技术的垄断，将公司打造成为科研工具和诊断原料的国内领导品牌，乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台，同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台，我们对每一支自产的抗体进行了严格的检测。当然，我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势：1，严自检，保质量；2产品多，指标全；3，价格低，货期短。注：ABclonal抗体价格体系详情见附件
ABclonal抗体成分在平时工作当中，常会有客户咨询我们的抗体用的什么buffer进行保存，一般来说，我们的buffer的成分是：PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油；也有一些是PBS含0.03%的proclin300，50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮（MCI）和5-氯-2-甲基-4-异噻唑啉-3-酮（CMCI）。ProClin生物灭活剂能够迅速穿透细胞膜，抑制对细胞呼吸至关重要的特定酶，因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。
