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ABflo® 488 Rabbit anti-Human CD4 mAb (A22773)

Flow cytometry: 1X10^6 PC-3 cells (negative control,left) and THP-1 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD4 mAb (A22773,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 THP-1 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD4 mAb (A22773,5 μl/Test,right).

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 488 Rabbit anti-Human CD4 mAb (A22773,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained Human PBMC were used as blank control (red line).

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD4 mAb (A22773,5 μl/Test,right).

All(4)|
货号: A22773
促销价:   ¥620
货    期:现货产品
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详细信息

推荐稀释比
  • FC5 μl per 10^6 cells in 100 μl volume
浓度查询

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反应物种
Human
同种型
IgG
克隆号
ARC5142-ABf488
产品形式
Liquid
偶联物
ABflo® 488. Ex:491nm. Em:516nm.
存储缓冲液
Store at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300,0.2% BSA,pH7.3.
细胞定位
Cell membrane,Single-pass type I membrane protein
纯化方式
Affinity purification

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用户验证的应用

应用及物种
  • FC (Mus musculus)

客户数据及评论 (4)

折叠内容
CustomerMar 252024
评分
Experiment Type
FC
Sample
Human PBMCs
Description
Using ABflo® 488 Rabbit anti-Human/Monkey CD4 and ABflo® 647 Rabbit anti-Human HLA-DR monoclonal antibody for flow cytometry analysis of human PBMCs, the results showed a clear separation of cell populations.
CustomerMar 252024
评分
Experiment Type
FC
Sample
Human PBMCs
Description
Flow cytometry analysis of human PBMCs using ABflo® 488 Rabbit anti-Human/Monkey CD4 and ABflo® 647 Rabbit anti-Human TIGIT monoclonal antibody clearly classified positive and negative cells.
CustomerJan 222024
评分
Experiment Type
FC
Sample
Human PBMC
Description
Flow cytometry analysis of human PBMC using ABflo®488 Rabbit anti-Human/Monkey CD4 monoclonal antibody clearly classified positive and negative cells.
CustomerDec 152023
评分
Experiment Type
FC
Sample
Human PBMCs
Description
Flow cytometry analysis of human PBMCs using ABflo® 594 Rabbit anti-Human CD62L/L-Selectin mAb (A24632), PE Mouse anti-Human CD3 mAb (A22795), ABflo® 488 Rabbit anti-Human/Monkey CD4 mAb (A22773), APC Rabbit anti-Human/Monkey CD8a mAb (A24799) and ABflo® 594 Rabbit anti-Human CD38 mAb (A24366) clearly classified positive and negative cells.

文献引用 (1)

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Publishing research using A22773? Please let us know so that we can cite the reference in this datasheet.

背景信息

This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

基因ID
Swiss Prot
别名
T4; IMD79; Leu-3; OKT4D; CD4mut

抗原信息

免疫原
Recombinant protein of human CD4.

关联靶点

CD4为关键词搜索文献记录,得到上述基因与CD4的研究紧密相关,排序顺序根据研究相关程度由高到低决定。(所显示数字代表两者共同出现的已发表文献数量)
1/

关联领域

CD4为关键词搜索文献记录,得到上述相关热词信息,可反映出CD4的研究趋势与热点。(关联领域与基因名称的距离代表其研究热度)

* 该数据来源于赛特新思(citexs.com)
来自于发表文献,仅供参考所用,ABclonal不承担任何责任。

暂无以CD4为关键词搜索的文献记录。

该服务由赛特新思提供,上述关联分析来自已发表文献,仅供参考所用,ABclonal不承担任何责任。

ABclonal公司的抗体,可以回收利用几次?

首先,一般抗体不推荐客户回收利用,抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,ABclonal公司也做过一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定。
注:我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存,效价高的抗体可至少保存1周,至少重复利用3次。

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ABclonal作为国产抗体品牌的优势?

武汉爱博泰克生物(ABclonal)科技有限公司是国产品牌,她成立于2011年,公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心,凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士,组成我们的科学家团队,通过聚焦抗体与酶核心技术,致力于打破国际技术的垄断,将公司打造成为科研工具和诊断原料的国内领导品牌,乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台,同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台,我们对每一支自产的抗体进行了严格的检测。当然,我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势:1,严自检,保质量;2产品多,指标全;3,价格低,货期短。注:ABclonal抗体价格体系详情见附件

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ABclonal抗体成分?

ABclonal抗体成分在平时工作当中,常会有客户咨询我们的抗体用的什么buffer进行保存,一般来说,我们的buffer的成分是:PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油;也有一些是PBS含0.03%的proclin300,50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮(MCI)和5-氯-2-甲基-4-异噻唑啉-3-酮(CMCI)。ProClin生物灭活剂能够迅速穿透细胞膜,抑制对细胞呼吸至关重要的特定酶,因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。

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实验方案

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