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TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

文献引用 (85) 评论 (11) 说明书 收藏

Western blot analysis of various lysates using TriMethyl-Histone H3-K27 Rabbit pAb (A2363) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 0.5s.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (A2363).

Immunohistochemistry analysis of paraffin-embedded Human testis using TriMethyl-Histone H3-K27 Rabbit pAb (A2363) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunofluorescence analysis of PC-12 cells using TriMethyl-Histone H3-K27 Rabbit pAb (A2363) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K27 antibody (A2363) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K27 Rabbit pAb antibody (A2363) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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货号: A2363
促销价：   ￥1260
货    期：现货产品
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详细信息

推荐稀释比
  • WB1:500 - 1:1000
  • DB1:500 - 1:1000
  • IHC-P1:50 - 1:200
  • IF/ICC1:50 - 1:200
  • IP0.5ug-4ug antibody for 400ug-600ug extracts of whole cells
  • ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP5μg antibody for 5μg-10μg of Chromatin
  • ChIP-seq1:20 - 1:100
浓度查询

请输入产品标签上的lot号，例如4000000001

反应物种
Human, Mouse, Rat, Other (Wide Range Predicted)
同种型
IgG
理论分子量
16kDa
实际分子量
17kDa
产品形式
Liquid
偶联物
Unconjugated
存储缓冲液
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
阳性样本
MCF7 acid extract,293T acid extract,C2C12 acid extract,NIH/3T3 acid extract,C6 acid extract,H3 protein
细胞定位
Chromosome,Nucleus.
纯化方式
Affinity purification

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用户验证的应用

应用及物种
  • IF (Human embryonic stem cells , Mouse E16.5 neocortex , Goat sperm , HEK293T , RiceDrosophila melanogaster , Mus musculus , Gallus gallus , Sus scrofa , Homo sapiens , Danio rerio)
  • ChIP (A549 , Mouse embryonic stem cells , HeLa , Human , RiceGossypium , Oryza sativa , Other , Mus musculus , Arabidopsis thaliana , Gossypium , Ricinus communis , Homo sapiens , Homo sapiens , Mus musculus , Rattus norvegicus , Homo sapiens , Mus musculus , Danio rerio)
  • IHC (Mouse lung tumorMus musculus , Mus musculus , Homo sapiens , Mus musculus , Picea abies , Sus scrofa , Homo sapiens)
  • WB (Mus musculus , Homo sapiens , Sus scrofa , Drosophila , Eurosta solidaginis , Homo sapiens , Mus musculus , Rattus norvegicus , Monascus ruber , Danio rerio)
  • ChIP-Seq (Zea mays , Triticum aestivum , Oryza sativa , Homo sapiens , Other)
  • IP (Gossypium , Arabidopsis thaliana , Mus musculus , Homo sapiens)
  • Co-IP (N. benthamiana)
  • CUT&RUN (Mus musculus)
  • CUT&Tag (Mus musculus)
  • qPCR (Mus musculus)

客户数据及评论 (11)

折叠内容
CustomerAug 222018
评分
Experiment Type
Immunofluorescence (IF)
Sample
Human embryonic stem cells
Description
Immunofluorescence analysis of Human embryonic stem cells using antibody at 1:200 dilution.
CustomerMay 312017
评分
Experiment Type
Chromatin Immunoprecipitation(ChIP)
Sample
A549
Description
Chromatin Immunoprecipitation analysis of A549 cells, using TriMethyl-Histone H3-K27 Polyclonal Antibody.
CustomerDec 252016
评分
Experiment Type
Chromatin Immunoprecipitation(ChIP)
Sample
Mouse embryonic stem cells
Description
Chromatin Immunoprecipitation analysis of Mouse embryonic stem cells, using TriMethyl-Histone H3-K27 Polyclonal Antibody.
CustomerMay 112015
评分
Experiment Type
Immunohistochemistry(IHC)
Sample
Mouse lung tumor
Description
Immunohistochemistry analysis of Mouse lung tumor, using TriMethyl-Histone H3-K27 Polyclonal Antibody.
CustomerMay 112015
评分
Experiment Type
Immunofluorescence(IF)
Sample
Mouse E16.5 neocortex
Description
Immunofluorescence analysis of Mouse E16.5 neocortex, using TriMethyl-Histone H3-K27 Polyclonal Antibody.
CustomerMay 102015
评分
Experiment Type
Immunofluorescence(IF)
Sample
Goat sperm
Description
Immunofluorescence analysis of Goat sperm, using TriMethyl-Histone H3-K27 Polyclonal Antibody.
CustomerMay 082015
评分
Experiment Type
Immunofluorescence(IF)
Sample
HEK293T
Description
Immunofluorescence analysis of HEK293T cells, using TriMethyl-Histone H3-K27 Polyclonal Antibody.
CustomerMay 062015
评分
Experiment Type
Chromatin Immunoprecipitation(ChIP)
Sample
HeLa
Description
MYT-1 is the target gene of H3K27me3.
CustomerMay 052015
评分
Experiment Type
Chromatin Immunoprecipitation(ChIP)
Sample
Human
Description
A pair of cancerous tissues, prostate cancer (786818-T) and pericarcinomatous (786818-N) were selected to detect the enrichment degree of antibody by chip-qpcr (H3K27me3 can combine with MYOD1 specifically, but not whit SAT2).Results: The concentration of DNA was higher than 10ng/ul after chip (In the previous test, the DNA which had been pulled down with milliopre antibody was not detected out). The enrichment degree was five to eight times, and the specificity of antibody was good (nonspecific enrichment was not happen with SAT2).
CustomerMay 042015
评分
Experiment Type
Chromatin Immunoprecipitation(ChIP)
Sample
Rice
Description
Use Nipponbare callus (same quantity, about 3g) as sample material of ChIP experiment, 02g and 03g as target gene. Conduct IP experiment using Anti-H3K27me3 (millipore) and Anti-H3K27me3 (abclone). See result as below (The experiment result has been listed below).
CustomerMay 042015
评分
Experiment Type
Immunofluorescence(IF)
Sample
Rice
Description
Immunofluorescence analysis of Rice cells, using TriMethyl-Histone H3-K27 Polyclonal Antibody.

文献引用 (85)

折叠内容

Publishing research using A2363? Please let us know so that we can cite the reference in this datasheet.

    背景信息

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

    RRID
    AB_2756439
    基因ID
    8290
    8350
    Swiss Prot
    Q16695
    P68431
    别名
    H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; TriMethyl-Histone H3-K27

    抗原信息

    免疫原
    A synthetic peptide corresponding to a sequence within amino acids 1-100 of human histone H3 (NP_003520.1).
    序列
    MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY
    进行序列比对
    研究领域

    关联靶点

    以H3-4为关键词搜索文献记录，得到上述基因与H3-4的研究紧密相关，排序顺序根据研究相关程度由高到低决定。（所显示数字代表两者共同出现的已发表文献数量）
    ◀1/▶

    关联领域

    以H3-4为关键词搜索文献记录，得到上述相关热词信息，可反映出H3-4的研究趋势与热点。（关联领域与基因名称的距离代表其研究热度）

    * 该数据来源于赛特新思(citexs.com)
    来自于发表文献，仅供参考所用，ABclonal不承担任何责任。

    暂无以H3-4为关键词搜索的文献记录。

    该服务由赛特新思提供，上述关联分析来自已发表文献，仅供参考所用，ABclonal不承担任何责任。

    FAQ

    折叠内容

    ABclonal公司的抗体，可以回收利用几次？

    首先，一般抗体不推荐客户回收利用，抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，ABclonal公司也做过一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定。
    注：我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存，效价高的抗体可至少保存1周，至少重复利用3次。

    展开

    ABclonal作为国产抗体品牌的优势？

    武汉爱博泰克生物（ABclonal）科技有限公司是国产品牌，她成立于2011年，公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心，凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士，组成我们的科学家团队，通过聚焦抗体与酶核心技术，致力于打破国际技术的垄断，将公司打造成为科研工具和诊断原料的国内领导品牌，乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台，同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台，我们对每一支自产的抗体进行了严格的检测。当然，我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势：1，严自检，保质量；2产品多，指标全；3，价格低，货期短。注：ABclonal抗体价格体系详情见附件

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    ABclonal抗体成分？

    ABclonal抗体成分在平时工作当中，常会有客户咨询我们的抗体用的什么buffer进行保存，一般来说，我们的buffer的成分是：PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油；也有一些是PBS含0.03%的proclin300，50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮（MCI）和5-氯-2-甲基-4-异噻唑啉-3-酮（CMCI）。ProClin生物灭活剂能够迅速穿透细胞膜，抑制对细胞呼吸至关重要的特定酶，因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。

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    实验方案

    折叠内容
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