Stable transfected mouse cell line overexpressing human Nectin-4
Description
Flow cytometry analysis of Stable transfected mouse cell line overexpressing human Nectin-4, using ABflo® 647 Rabbit IgG isotype control (A22070) and ABflo® 647 Rabbit anti-Human Nectin-4 mAb (A25193) at 1:40 dilution. clearly classified positive and negative cells.
Nectin-4/ poliovirus Receptor-like 4 (PVRL4) is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily that promotes intercellular adhesion by acting as a major component of adhesion junctions. The extracellular domain of Nectin-4 consists of one Ig variable region (V) and two Ig constant regions (C), which mediate binding to measles virus and adjacent cells through trans-heterophile interactions with Nectin-1. Unlike other members of the connectin family, which are widely expressed in adult tissues, human Nectin-4 expression is largely limited to the blastoderm. Studies have shown that Nectin-4 is overexpressed in cells from a variety of human solid tumors, including pancreatic, breast, lung, and ovarian cancers. The expression pattern of Nectin-4 in normal tissues is limited, so NECTIN-4 can be used as a novel therapeutic target for various human tumors.
