![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_WB_03.jpg?t=1722997492)
Western blot analysis of lysates from NIH/3T3 cells using [KO Validated] HDAC1 Rabbit mAb (A26492) at 1:25000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_WB_04.jpg?t=1722997492)
Western blot analysis of lysates from wild type (WT) and HDAC1 knockout (KO) 293T cells using [KO Validated] HDAC1 Rabbit mAb (A26492) at 1:25000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_IHC_01.jpg?t=1722997492)
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_IHC_02.jpg?t=1722997492)
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_IHC_03.jpg?t=1722997492)
Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_IHC_04.jpg?t=1722997492)
Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_IHC_05.jpg?t=1722997492)
Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_IHC_06.jpg?t=1722997492)
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_IHC_07.jpg?t=1722997492)
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_IF_01.jpg?t=1722997492)
Confocal imaging of NIH/3T3 cells using [KO Validated] HDAC1 Rabbit mAb (A26492, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_IF_02.jpg?t=1722997492)
Confocal imaging of MCF7 cells using [KO Validated] HDAC1 Rabbit mAb (A26492, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_IF_03.jpg?t=1722997492)
Confocal imaging of HeLa cells using [KO Validated] HDAC1 Rabbit mAb (A26492, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_IF_04.jpg?t=1722997492)
Confocal imaging of paraffin-embedded Human colon cancer tissue using [KO Validated] HDAC1 Rabbit mAb (A26492, dilution 1:4000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A26492_ARC70626-01_IP_01.jpg?t=1722997492)
Immunoprecipitation of HDAC1 from 300 µg extracts of 293T cells was performed using 0.5 µg of [KO Validated] HDAC1 Rabbit mAb (A26492). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1 : 5000.