Flow cytometric analysis of mouse spleen tissue was performed using the following antibody panel: PE-conjugated rat anti-mouse Ly-6G (A27159), PE/Cyanine7-conjugated rabbit anti-mouse CD11b (A27339), APC/Cyanine7-conjugated rabbit anti-mouse CD45 (A26831), ABflo® 488-conjugated rat anti-mouse CD3 (A27161), ABflo® 647-conjugated rabbit anti-mouse CD8a (A24942), and ABflo® 500-conjugated rabbit anti-mouse CD4 (A27843). The analysis demonstrated clear separation between positive and negative populations within both T lymphocyte and neutrophil compartments.
Flow cytometric analysis of mouse heart tissue was performed using the following antibody panel: PE-conjugated rat anti-mouse Ly-6G (A27159), PE/Cyanine7-conjugated rabbit anti-mouse CD11b (A27339), APC/Cyanine7-conjugated rabbit anti-mouse CD45 (A26831), ABflo® 488-conjugated rat anti-mouse CD3 (A27161), ABflo® 647-conjugated rabbit anti-mouse CD8a (A24942), and ABflo® 500-conjugated rabbit anti-mouse CD4 (A27843). The analysis demonstrated clear separation between positive and negative populations within both T lymphocyte and neutrophil compartments.
Enables several functions, including heparan sulfate proteoglycan binding activity; heparin binding activity; and protein tyrosine phosphatase activity. Involved in several processes, including lymphocyte differentiation; positive regulation of macromolecule metabolic process; and regulation of signal transduction. Acts upstream of or within several processes, including lymphocyte differentiation; positive regulation of lymphocyte activation; and regulation of protein phosphorylation. Located in external side of plasma membrane; focal adhesion; and membrane raft. Is expressed in several structures, including 3rd branchial arch; alimentary system; cardiovascular system; hemolymphoid system; and placenta. Used to study systemic lupus erythematosus. Human ortholog(s) of this gene implicated in hepatitis C; multiple sclerosis; severe combined immunodeficiency; and severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-positive, Nk cell-positive. Orthologous to human PTPRC (protein tyrosine phosphatase receptor type C).
