Flow cytometric analysis of mouse spleen tissue was performed using the following antibody panel: PE-conjugated rat anti-mouse Ly-6G (A27159), PE/Cyanine7-conjugated rabbit anti-mouse CD11b (A27339), APC/Cyanine7-conjugated rabbit anti-mouse CD45 (A26831), ABflo® 488-conjugated rat anti-mouse CD3 (A27161), ABflo® 647-conjugated rabbit anti-mouse CD8a (A24942), and ABflo® 500-conjugated rabbit anti-mouse CD4 (A27843). The analysis demonstrated clear separation between positive and negative populations within both T lymphocyte and neutrophil compartments.
Flow cytometric analysis of mouse heart tissue was performed using the following antibody panel: PE-conjugated rat anti-mouse Ly-6G (A27159), PE/Cyanine7-conjugated rabbit anti-mouse CD11b (A27339), APC/Cyanine7-conjugated rabbit anti-mouse CD45 (A26831), ABflo® 488-conjugated rat anti-mouse CD3 (A27161), ABflo® 647-conjugated rabbit anti-mouse CD8a (A24942), and ABflo® 500-conjugated rabbit anti-mouse CD4 (A27843). The analysis demonstrated clear separation between positive and negative populations within both T lymphocyte and neutrophil compartments.
Flow Cytometry analysis of mouse liver , using PE Rabbit anti-Mouse CD161c/NK1.1 mAb (A24107) and ABflo® 488 Rat anti-Mouse CD3 mAb (A27161) at a dilution of 1:25.
Flow cytometry analysis of mouse liver tissue using ABflo® 488 Rat anti-Mouse CD3 mAb (A27161) and PE Rabbit anti-Mouse CD161c/NK1.1 mAb (A24107) at 1:20 dilution. clearly classified positive and negative cells.
Predicted to enable identical protein binding activity and transmembrane signaling receptor activity. Involved in positive thymic T cell selection. Part of alpha-beta T cell receptor complex. Is expressed in thymus primordium. Human ortholog(s) of this gene implicated in immunodeficiency 19 and severe combined immunodeficiency. Orthologous to human CD3D (CD3d molecule). Predicted to enable several functions, including SH3 domain binding activity; identical protein binding activity; and protein heterodimerization activity. Involved in nervous system development and positive regulation of cell adhesion. Acts upstream of or within several processes, including positive regulation of T cell activation; positive regulation of cytokine production; and regulation of signal transduction. Located in several cellular components, including dendritic spine; external side of plasma membrane; and immunological synapse. Part of alpha-beta T cell receptor complex. Is expressed in colon and hemolymphoid system. Human ortholog(s) of this gene implicated in immunodeficiency 18. Orthologous to human CD3E (CD3e molecule).
