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PPAT Polyclonal Antibody

概述
货号: A6698
宿主: Rabbit
同种型: IgG
ABclonal:Western blot - PPAT Polyclonal Antibody (A6698)
ABclonal:Immunofluorescence - PPAT Polyclonal Antibody (A6698)
Review
ABclonal: review for PPAT Polyclonal Antibody(A6698)
优惠价
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背景

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.

免疫原信息

免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPAT (NP_002694.3).
序列MELEELGIREECGVFGCIASGEWPTQLDVPHVITLGLVGLQHRGQESAGIVTSDGSSVPTFKSHKGMGLVNHVFTEDNLKKLYVSNLGIGHTRYATTGKCELENCQPFVVETLHGKIAVAHNGELVNAARLRKKLLRHGIGLSTSSDSEMITQLLAYTPPQEQDDTPDWVARIKNLMKEAPTAYSLLIMHRDVIYAVRDPYGNRPLCIGRLIPVSDINDKEKKTSETEGWVVSSESCSFLSIGARYYREVLPGEIVEISRHNVQTLDIISRSEGNPVAFC
基因ID5471
Swiss ProtQ06203
别名PPAT; ATASE; GPAT; PRAT; amidophosphoribosyltransferase

应用

反应物种Human, Mouse, Rat, Monkey
验证应用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
用户验证的应用
推荐稀释比
WB 1:500 - 1:2000
IF 1:50 - 1:100
理论分子量57kDa
实际分子量55kDa
存储缓冲液Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
实验方法Western blotting    
阳性样本HepG2,COS-7
细胞定位
纯化方式Affinity purification

PPAT Polyclonal Antibody 检测图片

ABclonal:Western blot - PPAT Polyclonal Antibody (A6698) }

Western blot - PPAT Polyclonal Antibody (A6698)

Western blot analysis of extracts of various cell lines, using PPAT antibody (A6698) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - PPAT Polyclonal Antibody (A6698) }

Immunofluorescence - PPAT Polyclonal Antibody (A6698)

Immunofluorescence analysis of HeLa cells using PPAT Polyclonal Antibody (A6698) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

评论列表

CustomerMar 232016
Review for PPAT Polyclonal Antibody(A6698)5
Cat.No
A6698
Application
Western blot(WB)
Sample
Rat liver
Loading Amount
8 ug
Dilution
1:20000
Incubatition time
over night
Temperature
4 ℃
Exposure
30s
Observed Band
57 kDa
Remarks
Western blot analysis of extracts of Rat liver, using PPAT Polyclonal Antibody.

More

>

Reviews List

CustomerMar 232016
Review for PPAT Polyclonal Antibody(A6698)5
Cat.No
A6698
Application
Western blot(WB)
Sample
Rat liver
Loading Amount
8 ug
Dilution
1:20000
Incubatition time
over night
Temperature
4 ℃
Exposure
30s
Observed Band
57 kDa
Remarks
Western blot analysis of extracts of Rat liver, using PPAT Polyclonal Antibody.

More

>

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