概述
货号: AC001
宿主: Rabbit
同种型: IgG
ABclonal:Western blot - GAPDH Polyclonal Antibody (AC001)
ABclonal:Immunohistochemistry - GAPDH Polyclonal Antibody (AC001)
ABclonal:Immunohistochemistry - GAPDH Polyclonal Antibody (AC001)
ABclonal:Immunohistochemistry - GAPDH Polyclonal Antibody (AC001)
ABclonal:Immunohistochemistry - GAPDH Polyclonal Antibody (AC001)
ABclonal:Immunofluorescence - GAPDH Polyclonal Antibody (AC001)
Review
ABclonal: review for GAPDH Polyclonal Antibody(AC001)
优惠价
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背景

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

免疫原信息

免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (NP_002037.2).
序列MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
基因ID2597
Swiss ProtP04406
别名GAPDH; G3PD; GAPD; HEL-S-162eP; glyceraldehyde-3-phosphate dehydrogenase

应用

反应物种Human, Mouse, Rat
验证应用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
用户验证的应用

WB (Human , Mouse , Rat , Piglets)

推荐稀释比
WB 1:1000 - 1:10000
IHC 1:50 - 1:100
IF 1:20 - 1:50
IP 1:20 - 1:50
理论分子量31kDa/36kDa
实际分子量36kDa
存储缓冲液Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
实验方法Western blotting    
阳性样本PC-3,HepG2,293T,HeLa,Mouse brain,Mouse heart,Mouse skeletal muscle
细胞定位Cytoplasm,Membrane,Nucleus,cytoskeleton,cytosol,perinuclear region
纯化方式Affinity purification

GAPDH Polyclonal Antibody 检测图片

ABclonal:Western blot - GAPDH Polyclonal Antibody (AC001) }

Western blot - GAPDH Polyclonal Antibody (AC001)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (AC001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - GAPDH Polyclonal Antibody (AC001) }

Immunohistochemistry - GAPDH Polyclonal Antibody (AC001)

Immunohistochemistry of paraffin-embedded rat pancreas using GAPDH antibody (AC001) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GAPDH Polyclonal Antibody (AC001) }

Immunohistochemistry - GAPDH Polyclonal Antibody (AC001)

Immunohistochemistry of paraffin-embedded human breast cancer using GAPDH antibody (AC001) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GAPDH Polyclonal Antibody (AC001) }

Immunohistochemistry - GAPDH Polyclonal Antibody (AC001)

Immunohistochemistry of paraffin-embedded mouse heart using GAPDH antibody (AC001) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GAPDH Polyclonal Antibody (AC001) }

Immunohistochemistry - GAPDH Polyclonal Antibody (AC001)

Immunohistochemistry of paraffin-embedded mouse heart using GAPDH antibody (AC001) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - GAPDH Polyclonal Antibody (AC001) }

Immunofluorescence - GAPDH Polyclonal Antibody (AC001)

Immunofluorescence analysis of MCF-7 cells using GAPDH antibody (AC001). Blue: DAPI for nuclear staining.

评论列表

CustomerAug 192018
Review for GAPDH Polyclonal Antibody(AC001)5
Cat.No
AC001
Application
Western blot (WB)
Sample
MSC cells
Loading Amount
15 ug
Dilution
1:1000
Incubatition time
over night
Temperature
4 ℃
Exposure
30s
Observed Band
40 kDa
Remarks
Western blot analysis of extracts of rat cells, using antibody at 1:1000 dilution.

More

>

Reviews List

CustomerAug 192018
Review for GAPDH Polyclonal Antibody(AC001)5
Cat.No
AC001
Application
Western blot (WB)
Sample
MSC cells
Loading Amount
15 ug
Dilution
1:1000
Incubatition time
over night
Temperature
4 ℃
Exposure
30s
Observed Band
40 kDa
Remarks
Western blot analysis of extracts of rat cells, using antibody at 1:1000 dilution.

More

>

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