概述
货号: AC006
宿主: Rabbit
同种型: IgG
ABclonal:Western blot - ACTB Polyclonal Antibody (AC006)
ABclonal:Immunofluorescence - ACTB Polyclonal Antibody (AC006)
Review
ABclonal: review for ACTB Polyclonal Antibody(AC006)
优惠价
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背景

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

免疫原信息

免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human ACTB (NP_001092.1).
序列MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
基因ID60
Swiss ProtP60709
别名ACTB; BRWS1; PS1TP5BP1; beta actin

应用

反应物种Human, Mouse, Rat
验证应用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
用户验证的应用

WB (Human , C. gigas , Rat , human , Rat)

IF (human,Rat)

推荐稀释比
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
理论分子量41kDa
实际分子量42kDa
存储缓冲液Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
实验方法Western blotting    
阳性样本THP-1,293T,A-431,Mouse lung,Mouse lung,Mouse spleen,Mouse kidney,Mouse thymus
细胞定位Cytoplasm,cytoskeleton
纯化方式Affinity purification

ACTB Polyclonal Antibody 检测图片

ABclonal:Western blot - ACTB Polyclonal Antibody (AC006) }

Western blot - ACTB Polyclonal Antibody (AC006)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC006) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - ACTB Polyclonal Antibody (AC006) }

Immunofluorescence - ACTB Polyclonal Antibody (AC006)

Immunofluorescence analysis of HeLa cells using ACTB antibody (AC006) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

评论列表

CustomerSep 022018
Review for ACTB Polyclonal Antibody(AC006)5
Cat.No
AC006
Application
Western blot (WB)
Sample
MSC cells
Loading Amount
15 ug
Dilution
1:1000
Incubatition time
over night
Temperature
4 ℃
Exposure
10s
Observed Band
42 kDa
Remarks
Western blot analysis of extracts of MSC cells, using antibody at 1:1000 dilution.

More

>

Reviews List

CustomerSep 022018
Review for ACTB Polyclonal Antibody(AC006)5
Cat.No
AC006
Application
Western blot (WB)
Sample
MSC cells
Loading Amount
15 ug
Dilution
1:1000
Incubatition time
over night
Temperature
4 ℃
Exposure
10s
Observed Band
42 kDa
Remarks
Western blot analysis of extracts of MSC cells, using antibody at 1:1000 dilution.

More

>

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