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Plant actin Monoclonal Antibody

概述
货号: AC009
宿主: Mouse
同种型: IgG
ABclonal:Western blot - Plant actin Monoclonal Antibody (AC009)
ABclonal:Western blot - Plant actin Monoclonal Antibody (AC009)
Review
ABclonal: review for Plant actin Monoclonal Antibody(AC009)
优惠价
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背景

免疫原信息

免疫原Recombinant Protein of plants actin
序列Email for sequence
基因ID
Swiss Prot
别名

应用

反应物种Various Plants
验证应用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
用户验证的应用

WB (Poplar , unknown , Brassica napus , Arabidopsis thaliana)

推荐稀释比
WB 1:1000 - 1:2000
理论分子量45kDa
实际分子量45kDa
存储缓冲液Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
实验方法Western blotting    
阳性样本Arabidopsis thaliana tissue,Arabidopsis thaliana,Oryza sativa,Cassava,Zea mays
细胞定位
纯化方式Affinity purification

Plant actin Monoclonal Antibody 检测图片

ABclonal:Western blot - Plant actin Monoclonal Antibody (AC009) }

Western blot - Plant actin Monoclonal Antibody (AC009)

Western blot analysis of lysates from Arabidopsis thaliana tissue using plants actin antibody (AC009).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - Plant actin Monoclonal Antibody (AC009) }

Western blot - Plant actin Monoclonal Antibody (AC009)

Western blot analysis of extracts of various cell lines, using Plants actin antibody (AC009) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

评论列表

CustomerJul 052016
Review for Plant actin Monoclonal Antibody(AC009)5
Cat.No
AC009
Application
Western blot(WB)
Sample
Zea mays,Arabidopsis thaliana,Tomato
Loading Amount
30 ug
Dilution
1:2000
Incubatition time
over night
Temperature
4 ℃
Exposure
20s
Observed Band
unknow kDa
Remarks
We can detect right bands in Zea mays Arabidopsis thaliana and tomato samples.

More

>

Reviews List

CustomerJul 052016
Review for Plant actin Monoclonal Antibody(AC009)5
Cat.No
AC009
Application
Western blot(WB)
Sample
Zea mays,Arabidopsis thaliana,Tomato
Loading Amount
30 ug
Dilution
1:2000
Incubatition time
over night
Temperature
4 ℃
Exposure
20s
Observed Band
unknow kDa
Remarks
We can detect right bands in Zea mays Arabidopsis thaliana and tomato samples.

More

>

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