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Mouse anti GFP-Tag mAb (AE012)

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Western blot analysis of insect expressed GFP protein using Mouse anti GFP-Tag mAb (AE012) at different dilution.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.

Immunoprecipitation of SERPINB1-Flag-GFP from 300 µg extracts of 293T cells transfected with a SERPINB1 expression vector containing a single N-terminal Flag-GFP-Tag was performed using 3 µg of Mouse anti GFP-Tag mAb (AE012). Mouse Control IgG (AC011) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer.The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Rabbit anti DDDDK-Tag mAb (AE063) at a dilution of 1:5000.

Immunoprecipitation of SERPINB1-Flag-GFP from 300 µg extracts of 293T cells transfected with a SERPINB1 expression vector containing a single C-terminal Flag-GFP-Tag was performed using 3 µg of Mouse anti GFP-Tag mAb (AE012). Mouse Control IgG (AC011) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer.The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Rabbit anti DDDDK-Tag mAb (AE063) at a dilution of 1:5000.

All(3)|
货号: AE012

促销价：   ￥520
货    期：现货产品
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详细信息

推荐稀释比
  • WB1:10000 - 1:40000
  • IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
浓度查询

请输入产品标签上的lot号，例如4000000001

反应物种
Species independent
同种型
IgG1,Kappa
理论分子量
27kDa
实际分子量
27kDa/68kDa
克隆号
AMC0483R
产品形式
Liquid
偶联物
Unconjugated
存储缓冲液
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
阳性样本
insect expression of GFP,293T
纯化方式
Affinity purification

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用户验证的应用

应用及物种
  • IF (Homo sapiens , Mus musculus , Homo sapiens , Mus musculus , Homo sapiens , Mus musculus , Rattus norvegicus , Arabidopsis thaliana , Nicotiana tabacum , Drosophila melanogaster , Chlorocebus aethiops)
  • IP (Zea mays , Homo sapiens , Arabidopsis thaliana , Triticum aestivum , Nicotiana tabacum , N. benthamiana , Other , Ictalurus punctatus , Mus musculus , Homo sapiens , Chlorocebus aethiops , Anatinae)
  • Co-IP (Other , Oryza sativa , Stemphylium eturmiunum , Mus musculus , Homo sapiens , Arabidopsis thaliana , Malus domestica , Cucumis sativus , Glycine max , Agrobacterium , Helicoverpa armigera)
  • WB (Oryza sativa , Homo sapiens , Arabidopsis thaliana , Mus musculus , Other , G.hirsutum , Phytophthora capsici , Stemphylium eturmiunum , Chlorocebus aethiops , Saccharomyces cerevisiae , Glycine max , Caenorhabditis elegans , Nicotiana tabacum L. , Hordeum vulgare , Populus , Zea mays , Triticum aestivum , M. oryzae , Nicotiana benthamiana , N. tabacum , N. benthamiana , Homo sapiens , Mus musculus , Cynoglossus robustus , Ctenopharyngodon idellus , Pyrus pyrifolia , Helicoverpa armigera , Agrobacterium , Solanum lycopersicum L , Cotton bollworms , Juvenile tilapia , N. tabacum , Rattus norvegicus , Anatinae , Yeast , Nicotiana tabacum L , Tadarida brasiliensis , Chlorocebus aethiops , N. benthamiana plants , Ictalurus punctatus , Solanum tuberosum , Danio rerio , tobacco , Nicotiana tabacum , Rosa rugosa Thunb , Carassius auratus gibelio , Sus scrofa)
  • Pull-down (Arabidopsis thaliana)
  • CUT&Tag (Oryza sativa)
  • ChIP (Arabidopsis thaliana , Other)
  • IHC (Homo sapiens , Mus musculus , Rattus norvegicus)
  • Other (Triticum aestivum , Mus musculus , Homo sapiens)

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文献引用 (218)

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Publishing research using AE012? Please let us know so that we can cite the reference in this datasheet.

背景信息

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest. GFP can be introduced into animals or other species through transgenic techniques, and maintained in their genome and that of their offspring. To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells.

RRID
AB_2770402
别名
GFP;GFP tag;GFP-tag

抗原信息

免疫原
A synthetic peptide corresponding to a sequence within amino acids 1-100 to the N-terminus of GFP protein.
序列
MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFF
进行序列比对

FAQ

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ABclonal公司的抗体，可以回收利用几次？

首先，一般抗体不推荐客户回收利用，抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，ABclonal公司也做过一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定。
注：我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存，效价高的抗体可至少保存1周，至少重复利用3次。

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ABclonal作为国产抗体品牌的优势？

武汉爱博泰克生物（ABclonal）科技有限公司是国产品牌，她成立于2011年，公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心，凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士，组成我们的科学家团队，通过聚焦抗体与酶核心技术，致力于打破国际技术的垄断，将公司打造成为科研工具和诊断原料的国内领导品牌，乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台，同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台，我们对每一支自产的抗体进行了严格的检测。当然，我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势：1，严自检，保质量；2产品多，指标全；3，价格低，货期短。注：ABclonal抗体价格体系详情见附件

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ABclonal抗体成分？

ABclonal抗体成分在平时工作当中，常会有客户咨询我们的抗体用的什么buffer进行保存，一般来说，我们的buffer的成分是：PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油；也有一些是PBS含0.03%的proclin300，50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮（MCI）和5-氯-2-甲基-4-异噻唑啉-3-酮（CMCI）。ProClin生物灭活剂能够迅速穿透细胞膜，抑制对细胞呼吸至关重要的特定酶，因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。

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实验方案

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