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Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

文献引用 (67) 评论 (3) 说明书 收藏

Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at 1:400 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.

Western blot analysis of lysates from C6 cells using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at 1:400 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunofluorescence analysis of 293T(treated with UV) and 293T(untreated) cells using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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货号: AP0099

促销价：   ￥1260
货    期：现货产品
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详细信息

推荐稀释比
  • WB1:500 - 1:1000
  • IHC-P1:50 - 1:200
  • IF/ICC1:100 - 1:500
  • ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
浓度查询

请输入产品标签上的lot号，例如4000000001

反应物种
Human, Mouse, Rat
同种型
IgG
理论分子量
15kDa
实际分子量
17kDa
产品形式
Liquid
偶联物
Unconjugated
存储缓冲液
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
阳性样本
NIH/3T3 treated by UV,C6 treated by UV
细胞定位
Chromosome,Nucleus.
纯化方式
Affinity purification

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用户验证的应用

应用及物种
  • WB (U251 cells , N2AMus musculus , Homo sapiens , Mus musculus , Rattus norvegicus)
  • IF (Mouse bone marrowHomo sapiens , Mus musculus , Homo sapiens , Rattus norvegicus)
  • ChIP (Arabidopsis thaliana)
  • IHC (Homo sapiens)

客户数据及评论 (3)

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CustomerMar 272018
评分
Experiment Type
Western blot(WB)
Sample
U251 cells
Description
Western blot analysis of extracts of U251 cells, using antibody at 1:2500 dilution.
CustomerAug 012016
评分
Experiment Type
Western blot(WB)
Sample
N2A
Description
Western blot analysis of extracts of N2A cells, using Phospho-H2AFX-S139 Polyclonal Antibody.
CustomerJul 312016
评分
Experiment Type
Immunofluorescence(IF)
Sample
Mouse bone marrow
Description
Confocal immunofluoresent images of mouse bone morrow showing nuclear localization with p-H2A.x Antibody 1:100 (left

文献引用 (67)

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Publishing research using AP0099? Please let us know so that we can cite the reference in this datasheet.

背景信息

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

RRID
AB_2771168
基因ID
3014
Swiss Prot
P16104
别名
H2A.X; H2A/X; H2AFX; Phospho-Histone H2AX-S139

抗原信息

免疫原
A synthetic phosphorylated peptide around S139 of human Histone H2AX (NP_002096.1).
序列
QASQEY
进行序列比对
研究领域

关联靶点

以H2AX为关键词搜索文献记录，得到上述基因与H2AX的研究紧密相关，排序顺序根据研究相关程度由高到低决定。（所显示数字代表两者共同出现的已发表文献数量）
◀1/▶

关联领域

以H2AX为关键词搜索文献记录，得到上述相关热词信息，可反映出H2AX的研究趋势与热点。（关联领域与基因名称的距离代表其研究热度）

* 该数据来源于赛特新思(citexs.com)
来自于发表文献，仅供参考所用，ABclonal不承担任何责任。

暂无以H2AX为关键词搜索的文献记录。

该服务由赛特新思提供，上述关联分析来自已发表文献，仅供参考所用，ABclonal不承担任何责任。

FAQ

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ABclonal公司的抗体，可以回收利用几次？

首先，一般抗体不推荐客户回收利用，抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，ABclonal公司也做过一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定。
注：我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存，效价高的抗体可至少保存1周，至少重复利用3次。

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ABclonal作为国产抗体品牌的优势？

武汉爱博泰克生物（ABclonal）科技有限公司是国产品牌，她成立于2011年，公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心，凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士，组成我们的科学家团队，通过聚焦抗体与酶核心技术，致力于打破国际技术的垄断，将公司打造成为科研工具和诊断原料的国内领导品牌，乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台，同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台，我们对每一支自产的抗体进行了严格的检测。当然，我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势：1，严自检，保质量；2产品多，指标全；3，价格低，货期短。注：ABclonal抗体价格体系详情见附件

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ABclonal抗体成分？

ABclonal抗体成分在平时工作当中，常会有客户咨询我们的抗体用的什么buffer进行保存，一般来说，我们的buffer的成分是：PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油；也有一些是PBS含0.03%的proclin300，50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮（MCI）和5-氯-2-甲基-4-异噻唑啉-3-酮（CMCI）。ProClin生物灭活剂能够迅速穿透细胞膜，抑制对细胞呼吸至关重要的特定酶，因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。

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实验方案

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