产品和服务 / 产品类型 / 抗体产品 / 磷酸化抗体

重组兔单抗

Phospho-AKT-S473 Rabbit mAb (AP0637)

文献引用 (83) 评论 (2) 说明书 收藏

Western blot analysis of various lysates using Phospho-AKT1-S473 mAb (AP0637) at 1:1000 dilution. Both Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-AKT1-S473 mAb (AP0637) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-AKT1-S473 Rabbit mAb (AP0637) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-AKT1-S473 Rabbit mAb (AP0637) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse stomach using Phospho-AKT1-S473 Rabbit mAb (AP0637) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-AKT1-S473 Rabbit mAb (AP0637) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

All(6)|
货号: AP0637

促销价：   ￥1600
货    期：现货产品
抗体定制服务咨询 |
扫码下单
享受积分

详细信息

推荐稀释比
  • WB1:500 - 1:2000
  • IHC-P1:50 - 1:200
浓度查询

请输入产品标签上的lot号，例如4000000001

反应物种
Human, Mouse, Rat
同种型
IgG
理论分子量
56kDa
实际分子量
60kDa
克隆号
ARC0169
产品形式
Liquid
偶联物
Unconjugated
存储缓冲液
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
阳性样本
Jurkat treated by Calyculin A,NIH/3T3 treated by Calyculin A,C6 treated by Calyculin A
细胞定位
Cell membrane,Cytoplasm,Nucleus
纯化方式
Affinity purification

展开

用户验证的应用

应用及物种
  • IHC (Human lung cancer cellsHomo sapiens , Mus musculus , Homo sapiens , Rattus norvegicus , Sus scrofa)
  • WB (Human lung cancer cellsMus musculus , Mus musculus , Homo sapiens , Homo sapiens , Mus musculus , Sus scrofa , Rattus norvegicus , Rattus norvegicus , gallus domesticus , Oryctolagus cuniculus , Rattus norvegicus , Sus scrofa , Gallus gallus)
  • IF (Homo sapiens , Rattus norvegicus , Oryctolagus cuniculus)

客户数据及评论 (2)

折叠内容
CustomerNov 192018
评分
Experiment Type
Immunohistochemistry (IHC)
Sample
Human lung cancer cells
Description
Immunohistochemistry of paraffin-embedded Human lung cancer cells by using antibody at dilution of 1:50
CustomerOct 092018
评分
Experiment Type
Western blot (WB)
Sample
Human lung cancer cells
Description
Western blot analysis of extracts of Human lung cancer cells, using antibody at 1:1000 dilution.

文献引用 (83)

折叠内容

Publishing research using AP0637? Please let us know so that we can cite the reference in this datasheet.

背景信息

This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene.

RRID
AB_2770898
基因ID
207
Swiss Prot
P31749
别名
AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA; Phospho-AKT1-S473

抗原信息

免疫原
A synthetic phosphorylated peptide around S473 of human Akt1 (P31749).
序列
QFSYS
进行序列比对
研究领域

关联靶点

以AKT1为关键词搜索文献记录，得到上述基因与AKT1的研究紧密相关，排序顺序根据研究相关程度由高到低决定。（所显示数字代表两者共同出现的已发表文献数量）
◀1/▶

关联领域

以AKT1为关键词搜索文献记录，得到上述相关热词信息，可反映出AKT1的研究趋势与热点。（关联领域与基因名称的距离代表其研究热度）

* 该数据来源于赛特新思(citexs.com)
来自于发表文献，仅供参考所用，ABclonal不承担任何责任。

暂无以AKT1为关键词搜索的文献记录。

该服务由赛特新思提供，上述关联分析来自已发表文献，仅供参考所用，ABclonal不承担任何责任。

FAQ

折叠内容

ABclonal公司的抗体，可以回收利用几次？

首先，一般抗体不推荐客户回收利用，抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，ABclonal公司也做过一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定。
注：我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存，效价高的抗体可至少保存1周，至少重复利用3次。

展开

ABclonal作为国产抗体品牌的优势？

武汉爱博泰克生物（ABclonal）科技有限公司是国产品牌，她成立于2011年，公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心，凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士，组成我们的科学家团队，通过聚焦抗体与酶核心技术，致力于打破国际技术的垄断，将公司打造成为科研工具和诊断原料的国内领导品牌，乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台，同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台，我们对每一支自产的抗体进行了严格的检测。当然，我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势：1，严自检，保质量；2产品多，指标全；3，价格低，货期短。注：ABclonal抗体价格体系详情见附件

展开

ABclonal抗体成分？

ABclonal抗体成分在平时工作当中，常会有客户咨询我们的抗体用的什么buffer进行保存，一般来说，我们的buffer的成分是：PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油；也有一些是PBS含0.03%的proclin300，50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮（MCI）和5-氯-2-甲基-4-异噻唑啉-3-酮（CMCI）。ProClin生物灭活剂能够迅速穿透细胞膜，抑制对细胞呼吸至关重要的特定酶，因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。

展开

实验方案

折叠内容
>