ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. For high-ratio antibody dilutions (≥1:10000)，a sequential dilution method is strongly recommended to ensure measurement accuracy.
浓度查询
请输入产品标签上的lot号，例如4000000001
免疫原
This information is considered to be commercially sensitive.
实际分子量
10 kDa or above
产品形式
Liquid
偶联物
Unconjugated
实际分子量
10 kDa or above
产品形式
Liquid
偶联物
Unconjugated
阳性样本
A-431 treated with Calyculin A
存储缓冲液
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS, pH 7.3, containing 50% glycerol. Preserved with Proclin300 or sodium azide. May contain 0.05% BSA as specified on the Certificate of Analysis.
Publishing research using AP0893SP? Please let us know so that we can cite the reference in this datasheet.
背景信息
As a critical post-translational modification, phosphorylation plays important roles in regulating various biological processes,Serine/threonine phosphorylation is an important mechanism that is involved in the regulation of protein function. Protein phosphorylation is the most well-studied post translational modification (PTM), in which a phosphoryl group from adenosine triphosphate (ATP) is covalently attached to a serine (~86%), threonine (~12%), or tyrosine (~2%) by a kinase and removed by a phosphatase. Phosphorylation at other amino acids have also been reported. Phosphorylation can modify protein structure, function, and interactions. As such, phosphorylation plays a critical role in virtually all cellular processes in homeostasis and disease, including signal transduction, cell cycle, differentiation, proliferation, metabolism, motility, and death. Importantly, phosphorylation at different residues can cause different outcomes. For example, RAF1 is a kinase central to the MAPK pathway that is activated when it is phosphorylated at serine (S) or threonine (T) residues S259, S338, S340/341, T491, or S494. However, phosphorylation at S289/296/301 results in the inhibition of RAF1 kinase activity.
