HRP-conjugated Rabbit anti-Monkey IgE (Fc) mAb (AS098)

Dose response curve of HRP conjugated Rabbit Anti-Monkey IgE mAb measured by ELISA. 25 ng of Monkey IgE recombinant protein was coated in 384-well plate, blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Monkey IgE mAb in a 2 fold serial dilution from 1 μg/mL to 7.8 pg/mL, incubation was performed at room temperature for 1 hour. EC50 value ( 6.54×10^-2 μg/mL) were calculated using 4 parameter logistic regression.

Cross reactivity to various IgGs of HRP conjugated Rabbit Anti-Monkey IgE mAb was examined ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate. Then the plate was blocked and incubated with 25 µL of HRP conjugated Rabbit Anti-Monkey IgE mAb , 0.5 μg/mL and 0.125 μg/mL) at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Monkey IgE antibody has highly specific recognition of -Monkey IgE, while no or minimal cross reactivity to Monkey IgD, Monkey IgA, Human IgD and Mouse IgD.