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TSA Fluorescence Quadra Staining Plus Kit(Rabbit secondary antibody){多重荧光免疫组化染色,四标五色,兔二抗} (RK50027P)

货号: RK50027P
价格:   ¥1800
货    期:InStock
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产品信息

产品简介
  • Note: Paraffin tissue sections, frozen tissue sections, cell slides (Frozen sections and cell slides need to be used with RM02984P antibody elution buffer dedicated for multiplex fluorescence staining).

  • The primary principle of this product is based on Tyramide Signal Amplification (TSA) technology. The TSA principle utilizes the peroxidase reaction of tyramide (i.e., HRP catalyzes H₂O₂ to activate fluorescently labeled tyramide salts, forming covalent binding sites), generating a substantial enzymatic reaction. The reaction product binds to surrounding protein residues (including tryptophan, histidine, and tyrosine residues), resulting in the deposition of a large amount of fluorophore at the antigen-antibody binding site to achieve signal amplification. Simultaneously, through antibody stripping, multiple fluorescence staining can be achieved using different fluorescent tyramides.
产品特点
  • 1.High Sensitivity:Based on Tyramide Signal Amplification (TSA) technology, it offers ultra-high sensitivity and provides more accurate detection results.
  • 2.Cost-Effective:Unlike traditional immunofluorescence, TSA allows the use of primary antibodies from the same or different species. Secondary antibodies are HRP-conjugated, and multiple dyes can be used for multiplex protein labeling.
  • 3.High Stability:The fluorescence is resistant to quenching, ensuring extremely high product stability.
产品应用
Suitable for multiplex immunofluorescence experiments on paraffin sections,frozen sections,and cell slides.
产品组成
Product CodeProduct NameSize(10 T)Size(50 T)Storage Temperature
RK50027P-1 TYR-520 plus fluorochrome(200×) 5 μL (Concentrated type) 25 μL (Concentrated type) 2-8℃ , Protect from Light
RK50027P-2 TYR-570 plus fluorochrome(200×) 5 μL (Concentrated type) 25 μL (Concentrated type) 2-8℃ , Protect from Light
RK50027P-3 TYR-620 plus fluorochrome(200×) 5 μL (Concentrated type) 25 μL (Concentrated type) 2-8℃ , Protect from Light
RK50027P-4 TYR-690 plus fluorochrome(200×) 5 μL (Concentrated type) 25 μL (Concentrated type) 2-8℃ , Protect from Light
RK50027P-5 TSA buffer 3.2 mL 16 mL 2-8℃
RK50027P-6 Polymer-HRP goat anti-rabbit secondary antibody(Ready-to-use) 3.2 mL 16 mL 2-8℃
RK50027P-7 DAPI staining solution(Ready-to-use) 1 mL 5 mL 2-8℃ , Protect from Light
RK50027P-8 Antibody diluent 4 mL 20 mL 2-8℃
RK50027P-9 Peroxidase inhibitor solution 4 mL 20 mL 2-8℃ , Protect from Light
RK50027P-10 Antifade mounting medium 1 mL 5 mL 2-8℃

注意事项

  • 1.Please read the instruction manual carefully before starting the experiment.
  • 2.This product is for Research Use Only. Not for use in diagnostic procedures or any other clinical applications.
  • 3.For your safety and health, please wear a lab coat and disposable gloves when handling this product.

保存条件和运输

Store at 2-8℃ for 12 months.

说明书

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