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TSA Fluorescence Penta Staining Plus Kit(Rabbit secondary antibody){多重荧光免疫组化染色,五标六色,兔二抗} (RK50028P)

货号: RK50028P
价 格:  2500.00
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产品信息

产品简介
  • Note: Paraffin tissue sections, frozen tissue sections, cell slides (Frozen sections and cell slides need to be used with RM02984P antibody elution buffer dedicated for multiplex fluorescence staining).

  • The primary principle of this product is based on Tyramide Signal Amplification (TSA) technology. The TSA principle utilizes the peroxidase reaction of tyramide (i.e., HRP catalyzes H₂O₂ to activate fluorescently labeled tyramide salts, forming covalent binding sites), generating a substantial enzymatic reaction. The reaction product binds to surrounding protein residues (including tryptophan, histidine, and tyrosine residues), resulting in the deposition of a large amount of fluorophore at the antigen-antibody binding site to achieve signal amplification. Simultaneously, through antibody stripping, multiple fluorescence staining can be achieved using different fluorescent tyramides.
产品特点
  • 1.High Sensitivity:Based on Tyramide Signal Amplification (TSA) technology, it offers ultra-high sensitivity and provides more accurate detection results.
  • 2.Cost-Effective:Unlike traditional immunofluorescence, TSA allows the use of primary antibodies from the same or different species. Secondary antibodies are HRP-conjugated, and multiple dyes can be used for multiplex protein labeling.
  • 3.High Stability:The fluorescence is resistant to quenching, ensuring extremely high product stability.
产品应用
WB
产品组成
Product CodeProduct NameSize(10 T)Size(50 T)Storage Temperature
RK50028P-1 TYR-520 plus fluorochrome(200×) 5 μL(Concentrated type) 25 μL(Concentrated type) 2-8℃ ,Protect from Light
RK50028P-2 TYR-570 plus fluorochrome(200×) 5 μL(Concentrated type) 25 μL(Concentrated type) 2-8℃ ,Protect from Light
RK50028P-3 TYR-620 plus fluorochrome(200×) 5 μL(Concentrated type) 25 μL(Concentrated type) 2-8℃ ,Protect from Light
RK50028P-4 TYR-690 plus fluorochrome(200×) 5 μL(Concentrated type) 25 μL(Concentrated type) 2-8℃ ,Protect from Light
RK50028P-5 TYR-480 plus fluorochrome(200×) 5 μL(Concentrated type) 25 μL(Concentrated type) 2-8℃ ,Protect from Light
RK50028P-6 TSA buffer 4 mL 20 mL 2-8℃
RK50028P-7 Polymer-HRP goat anti-rabbit secondary antibody(Ready-to-use) 4 mL 20 mL 2-8℃
RK50028P-8 DAPI staining solution(Ready-to-use) 1 mL 5 mL 2-8℃ ,Protect from Light
RK50028P-9 Antibody diluent 5 mL 25 mL 2-8℃
RK50028P-10 Peroxidase inhibitor solution 5mL 25 mL 2-8℃ ,Protect from Light
RK50028P-11 Antifade mounting medium 1 mL 5 mL 2-8℃

注意事项

  • 1.Please read the instruction manual carefully before starting the experiment.
  • 2.This product is for Research Use Only. Not for use in diagnostic procedures or any other clinical applications.
  • 3.For your safety and health, please wear a lab coat and disposable gloves when handling this product.

保存条件和运输

Store at 2-8℃ for 12 months.

说明书

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