DNMT1 Knockdown 293T Cell Lysate, Heterozygous (RM01774)

Genome sequence analysis of PCR products from parental (WT) and DNMT1 Knockdown (KD) 293T cells, using sanger sequencing.

All(1)|

货号: RM01774

详细信息

靶点

DNMT1

细胞系

293T

突变描述

DNMT1 Knockdown 293T Cell Line is engineered from 293T cell line with Gene-Editing technology.
Allele-1:50bp deletion in exon4
Allele-2:54bp deletion in exon4

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

基因ID

1786

Swiss Prot

P26358

别名

ADCADN; AIM; CXXC9; DNMT; HSN1E; MCMT; m.HsaI

研究领域