GSK3B Knockout HeLa Cell Lysate, Homozygous (RM01797)

Genome sequence analysis of PCR products from parental (WT) and GSK3B knockout (KO) HeLa cells, using sanger sequencing.

Western blot analysis of extracts from parental (Control) and GSK3B knockout (KO) HeLa cells, using GSK3B antibody (A3174) at 1:1000 dilution.

All(2)|

货号: RM01797

详细信息

靶点

GSK3B

细胞系

HeLa

突变描述

GSK3B Knockout HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:133bp deletion in exon2
Allele-2:134bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

基因ID

2932

Swiss Prot

P49841

研究领域