Genome sequence analysis of PCR products from parental (WT) and SIRT3 knockout (KO) 293T cells, using sanger sequencing.
Western blot analysis of extracts from parental (Control) and SIRT3 knockout (KO) 293T cells, using SIRT3 antibody (A5718) at 1:1000 dilution.
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
