EIF4EBP1 Knockdown HeLa Cell Lysate, Heterozygous (RM01856)

Genome sequence analysis of PCR products from parental (WT) and EIF4EBP1 Knockdown (KD) HeLa cells, using sanger sequencing.

All(1)|

货号: RM01856

详细信息

靶点

EIF4EBP1

细胞系

HeLa

突变描述

EIF4EBP1 Knockdown HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:116bp deletion in exon2
Allele-2:117bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

基因ID

1978

Swiss Prot

Q13541

别名

4E-BP1; 4EBP1; BP-1; PHAS-I

研究领域