CHRM1 Knockout HeLa Cell Line, Homozygous (RM01876)

Genome sequence analysis of PCR products from parental (WT) and CHRM1 knockout (KO) HeLa cells, using sanger sequencing.

All(1)|

货号: RM01876

详细信息

靶点

CHRM1

细胞系

HeLa

突变描述

CHRM1 Knockout HeLa Cell Line is engineered from HeLa cell line with Gene-Editing Technology.
Allele-1:137bp deletion in exon1
Allele-2:2bp deletion and 1bp insertion in exon1

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell line and 1 vial knockout cell line

细胞数目

1~5x10⁶ cells/vial

使用方法

Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37℃ with 5% CO₂ condition.
1. Thaw the vial in 37℃ water bath ,and shake it to melt as soon as possible.
2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me-
dium and centrifuge 1000rpm for approximately 5 minutes at room temperature.
3. Remove and discard the supernatant.
4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish.
5. Add 8-10mL of complete medium.
6. Incubate the culture at 37℃ incubator with 5% CO₂.
7. A subcultivation ratio of 1:2-1:4 is recommended.

物种

Human

保存条件

Stored in liquid nitrogen for a long time less than -130℃. Minimizing freeze-thaw cycles.

运输条件

Dry ice

背景信息

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13. [provided by RefSeq, Jul 2008]

基因ID

1128

Swiss Prot

P11229

别名

HM1; M1; M1R

研究领域